基于UPLC-MS/MS代谢组学技术探究乳杆菌对神经递质合成的影响OA

Objective:To investigate the effects of 2 different Lactobacillus species on peripheral neurotransmitters.Methods:A total of 18 8-week-old C57BL/6 male mice were selected for adaptive feeding for 1 week and randomly divided into the control(DZ),group the Lactobacillus reuteri(LYS)group,and the Lactobacillus acidophilus(SS)group,with 6 mice in each group.The DZ group was gavaged with 0.2 mL/each of saline per day,and the intervention groups(Lactobacillus reuteri and Lactobacil-lus acidophilus groups)were gavaged with 0.2 mL/each of lyophilized powdered solution of the corresponding strains per day,re-spectively.The blood of mice was collected after 10 weeks of gavage,and the neurotransmitters in mouse serum were analyzed qualitatively and quantitatively by UPLC-MS/MS.Meanwhile,the changes of neurotransmitter contents in the serum of mice in each group were observed by multivariate statistical analysis,and the differential metabolites were screened out by combining t-test(P<0.05)with OPLS-DA(VIP>1).Results:A total of 39 neurotransmitters from 5 classes were detected in mouse serum,in-cluding 21 amino acids,13 monoamines,1 choline,and 4 others.Multivariate statistical analysis revealed 9 differential metabo-lites.The content of ILA(P<0.01)and IAA(P<0.01)was significantly increased,and the content of GABA(P<0.05)was significantly decreased after the intervention by Lactobacillus reuteri and Lactobacillus acidophilus.Meanwhile,IAM(P<0.05),5-HIAA(P<0.05),and Pro(P<0.01)contents were found to be significantly higher,and Trp(P<0.01)and Gln(P<0.000 1)contents were significantly lower in the SS group after different Lactobacillus interventions,whereas XA(P<0.001)contents were found to be significantly higher in the LYS group.Conclusion:Both species of Lactobacillus can promote the release of excit-atory neurotransmitters and reduce the accumulation of inhibitory neurotransmitters,but there are some differences between the two species of Lactobacillus in the accumulation of other neurotransmitters,and the results of the study can provide theoretical and experimental bases for studies related to Lactobacillus to improve the disease.