基于ERK通路电针大鼠内关穴对糖尿病肢体缺血再灌注心肌损伤的研究OA

Objective:To investigate the effects of electroacupuncture at the Neiguan acupoint(PC6)on serum enzyme levels and the expressions of extracellular signal-regulated kinase(ERK)pathway proteins in a rat model of diabetic limb ischemia-reperfusion(IR),and to discuss its therapeutic mechanism.Methods:A total of 80 Sprague-Dawley(SD)rats were randomly divided into four groups:blank group,model group,electroacupuncture(EA)group,and Western medicine group.Diabetes models and limb ischemia-reperfusion(IR)models were established in the three groups except the blank group.Corresponding interventions were administered for seven days before and after the establishment of the limb IR model.Blood samples and cardiac tissue were collected from each group at different stages.To measure serum levels of creatine kinase-MB(CK-MB),cardiac troponin I(cTnI),and lactate dehydrogenase(LDH),and to detect the protein expression levels of protein kinase B(Akt),phosphorylated Akt(p-Akt),extracellular signal-regulated kinase 1/2(ERK1/2),and phosphorylated ERK1/2(p-ERK1/2)in myocardial tissue.Results:After limb IR modeling,compared with the blank group,the serum levels of CK-MB,cTnI,and LDH were increased in the other three groups(P<0.05).Compared with the model group,the serum myocardial enzyme levels were significantly decreased in the EA group and the Western medicine group(P<0.05).After seven days of continued intervention,compared with the model group,the serum levels of cTnI and LDH were significantly decreased in the EA group and the Western medicine group(P<0.05).However,there was no statistically significant difference in serum CK-MB level between the Western medicine group and the model group(P>0.05).After limb IR modeling,the ratios of p-Akt/Akt and p-ERK/ERK were increased in both the EA group and the Western medicine group(P<0.05),compared with the blank group,the p-Akt/Akt ratio was increased in the EA group(P<0.05),while it was decreased in the Western medicine group compared with the blank group(P<0.05).After seven days of continued intervention,compared with both the blank group and the model group,the EA group showed an increased p-Akt/Akt ratio(P<0.05)and a decreased p-ERK/ERK ratio(P<0.05).In contrast,the Western medicine group exhibited decreased ratios of both p-Akt/Akt and p-ERK/ERK compared with the blank group and the model group(P<0.05).Conclusion:Electroacupuncture at the Neiguan acupoint attenuates myocardial injury induced by diabetic limb IR,and its underlying mechanism may be associated with the upregulation of Akt and ERK signaling pathway activities.