罗汉果玉竹颗粒对脂多糖诱导大鼠急性肺损伤中Fibronectin、Collagen I及α-SMA表达的影响OA
Effects of Luohanguo Yuzhu Granules on the Expressions of Fibronectin,Collagen I and α-SMA in Rats with LPS-induced ALI
目的:研究罗汉果玉竹颗粒对脂多糖(lipopolysaccharide,LPS)诱导的大鼠急性肺损伤(acute lung injury,ALI)模型的保护作用及其可能机制.方法:将58只SPF(specific pathogen free,SPF)级Wistar大鼠按体质量随机分为空白对照组(K组,10只)、模型对照组(M组,12只)、罗汉果玉竹颗粒低剂量组(LY-L组,3.24 g·kg-1,12只)、罗汉果玉竹颗粒高剂量组(LY-H组,6.48 g·kg-1,12只)和泼尼松阳性药物对照组(PN组,5.4 mg·kg-1,12只).除K组外,其余各组大鼠均经尾静脉注射LPS溶液(6 mg·kg-1).造模后,各给药组每日灌胃相应药物1次,给药体积为10 mL·kg-1;K组和M组给予等体积蒸馏水.连续给药7天后,腹腔注射10％水合氯醛(3.5 mL·kg-1)麻醉,腹主动脉取血,采用酶联免疫吸附测定(enzyme-linked immu-nosorbent assay,ELISA)检测血清白细胞介素6(interleukin-6,IL-6)含量;取肺组织,苏木精-伊红(hema-toxylin-eosin,HE)染色观察病理变化,免疫组织化学法检测纤连蛋白(fibronectin,FN)、I型胶原(collagen I,COL1A1)及α平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的表达水平.结果:与K组比较,M组大鼠肺泡结构破坏,肺泡间隔明显增宽,肺泡壁大面积充血、水肿及炎性细胞浸润;血清IL-6含量升高(P<0.01),肺组织FN、COL1A1及α-SMA表达均增加(P<0.01).与M组比较,LY-L组和LY-H组大鼠血清IL-6含量降低(P<0.01),肺组织病理损伤明显改善,FN、COL1A1及α-SMA表达均降低(P<0.01).Pearson相关分析显示,血清IL-6含量与肺组织FN、COL1A1及α-SMA表达均呈正相关(P<0.01).结论:罗汉果玉竹颗粒对LPS诱导的大鼠ALI具有保护作用,其机制可能与减轻肺组织炎症反应、抑制IL-6释放,进而下调肺组织FN、COL1A1及α-SMA的表达有关.
Objective:To study the protective effects of Luohanguo(Siraitiae fructus)Yuzhu(Polygonati odorati rhizoma)granules on LPS-induced ALI in rats and its potential mechanism.Methods:All 58 SPF-grade Wistar rats were randomized into the blank control group(K group,10 rats),model control group(M group,12 rats),low-dose Luohanguo Yuzhu granules group(LY-L group,3.24 g·kg-1,12 rats),high-dose Luohanguo Yuzhu granules group(LY-H group,6.48 g·kg-1,12 rats)and prednisone positive control group(PN group,5.4 mg·kg-1,12 rats)according to body weight.Except for K group,the rats in the remaining groups received LPS solution(6 mg·kg-1)via tail vein injection.After successfully modeling,the treatment groups received the corresponding drugs by gavage once daily,administration volume was 10mL·kg-1;K group and M group were given the same volume of distilled water.After seven consecutive days of administration,the rats were anesthetized with an intraperitoneal injection of 10％chloral hydrate(3.5 mL·kg-1),and blood samples were collected from the abdominal aorta,ELISA was adopted to detect the contents of IL-6;lung tissues were collected for hematoxylin-eosin(HE)staining to observe pathological changes.Immunohistochemistry(IHC)was adopted to measure the expressions of FN,COL1A1 and α-SMA.Results:Compared with K group,M group observed disrupted alveolar structure,markedly thickened alveolar septa,and extensive congestion,edema,and inflammatory cell infiltration in the alveolar walls;an increase in the contents of IL-6(P<0.01),the elevated expressions of FN,COL1A1 and α-SMA in lung tissue(P<0.01).Compared with M group,LY-L group and LY-H group demonstrated a decrease in the contents of IL-6(P<0.01),a significant improvement in lung histopathological injury,the reduction in the expressions of FN,COL1A1 and α-SMA(P<0.01).Pearson correlation analysis showed that the contents of IL-6 were positively related to the expressions of FN,COL1A1 and α-SMA in lung tissue(P<0.01).Conclusion:Luohanguo Yuzhu granules have a protective effect against LPS-induced ALI in rats,and its mechanism might be attributed to the attenuation of the inflammatory response in lung tissue,the inhibition of IL-6 release,and the subsequent downregulation of FN,COL1A1,and α-SMA expression in the lungs.
赵丽华;康晓娜;韩小江
内蒙古自治区国际蒙医医院药学部,内蒙古 呼和浩特 010065内蒙古自治区国际蒙医医院药学部,内蒙古 呼和浩特 010065内蒙古医科大学附属医院,内蒙古 呼和浩特 010059
医药卫生
急性肺损伤罗汉果玉竹颗粒脂多糖纤连蛋白I型胶原α平滑肌肌动蛋白
acute lung injuryLuohanguo Yuzhu granuleslipopolysaccharidefibronectincollagen Iα-smooth muscle actin
《西部中医药》 2026 (3)
28-32,5
内蒙古自治区自然科学基金项目[2020MS(LH)0812].
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