不同浓度赤霉素及浸泡时间对豆科种子萌发的影响OA

To investigate the effects of gibberellin concentration and soaking time on the germination indices of four Legumi-nous species(Medicago ruthenica,Sophora alopecuroides,Astragalus mongholicus,and Medicago sativa),and to screen the optimal conditions for their seed germination.Seven gibberellin concentrations(0,100,200,300,400,500 mg/Land 600 mg/L)and four soaking durations(6,12,24 h and 36 h)were set,The petri dish paper germination method was employed to re-cord seed germination and calculate the germination percentage,germination energy,and germination index.The results showed that Medicago sativa exhibited the best germination performance,while Medicago ruthenica showed the poorest.For Medicago ruthenica,Astragalus mongholicus,and Medicago sativa,the germination indices generally followed a trend of ini-tial increase,subsequent decrease,and then another increase with increasing gibberellin concentration and soaking time.In contrast,the germination indices of Sophora alopecuroides showed a continuous decreasing trend under the same conditions.Under specific gibberellin concentration and soaking time treatments,the germination indices of all four leguminous species were improved.The optimal germination conditions were Medicago ruthenica with 400 mg/L gibberellin and 12 h soaking,So-phora alopecuroides with 100 mg/L gibberellin and 6 h soaking,Astragalus mongholicus with 200 mg/L gibberellin and 36 h soaking,and Medicago sativa with 400 mg/L gibberellin and 24 h soaking.