芦竹光合特性与染色体组型分析OA

To clarify the photosynthetic characteristics and chromosomal features of Arundo donax,a systematic identification of the photosynthetic pattern and karyotype of different Arundo donax germplasm materials was conducted.The photosynthetic type was com-prehensively determined through leaf anatomical structure observation(scanning electron microscopy),C4 photosynthetic enzyme ac-tivity assay,and carbon isotope ratio(δ13C)analysis,and the chromosome number and structural characteristics were cytogenetically identified using fluorescence in situ hybridization(FISH).The results showed that Arundo donax possessed a Kranz anatomy,exhibit-ing typical structural characteristics of a C4 plant.Both Lyuzhou 1 and Ninglu 1 showed clear 5S rDNA hybridization signals on four chromosomes,indicating a high conservation in the number of rDNA loci between the two materials.It was speculated that both Lyu-zhou 1 and Ninglu 1 might be tetraploids or aneuploids close to nonaploids.