苦豆子幼苗的功能性状对三种铜盐胁迫的响应OA

This study investigated the effects of three copper salts[CuSO4,Cu(CH3COO)2,and Cu(NO3)2]at dif-ferent concentration gradients(50,100,200,400,and 500 mg·L-1)on various functional traits of Sophora alope-curoides seedlings,aiming to provide a scientific basis for screening native plant resources for soil ecological res-toration in copper-contaminated areas.The results showed significant differences in the effects of different copper salts and their concentrations on the growth and physiological indicators of S.alopecuroides seedlings.Growth parameters of S.alopecuroides initially increased and subsequently decreased with the increase in stress intensi-ty—that is,low-concentration copper stress showed a growth-promoting effect,whereas high-concentration cop-per stress inhibited growth.Among the three salts,CuSO4 exhibited the highest inhibitory effect on the growth of S.alopecuroides.Under stress due to the three copper salts,the chlorophyll,soluble sugar,and soluble protein content in S.alopecuroides seedlings also initially increased and subsequently decreased with increasing salt con-centration.Superoxide dismutase(SOD)activity under Cu(CH3COO)2 treatment was significantly higher than un-der treatment with the other two copper salts.Pearson correlation analysis revealed a significant correlation among the growth and physiological indicators of S.alopecuroides seedlings under different copper salt treat-ments.The SOD content of Cu(NO3)2 was significantly positively correlated with soluble protein and soluble sugar.Fuzzy comprehensive evaluation showed that plants in the CuSO4(100 mg·L-1)treatment group showed high tolerance and could effectively maintain growth and physiological functions,whereas those in the CuSO4(500 mg·L-1)treatment group showed low tolerance.As a native plant,S.alopecuroides has high adaptability and tolerance and can play an important role as a pioneer or tolerant plant in copper-contaminated areas such as waste-lands and mining areas.