线粒体氧化应激相关基因表达与乳腺浸润性导管癌多模态超声特征具有相关性OA

Objective To elucidate the correlation between ultrasonographic features and mitochondrial oxidative stress-related gene expression in invasive ductal breast carcinoma.Methods A total of 38 patients diagnosed with invasive ductal carcinoma of the breast from January 2022 to August 2023 at the First Hospital of Shanxi Medical University were enrolled in this study.All participants had complete clinical records,multimodal ultrasound imaging data,and whole transcriptome sequencing profiles.To identify mitochondrial oxidative stress-related genes in breast cancer,we integrated differentially expressed genes from TCGA database with mitochondrial gene sets from MitoCarta 3.0 and oxidative stress-related genes from GeneCards.Key gene subgroups were then identified through Kaplan-Meier survival analysis,STRING protein-protein interaction network analysis,and Cytoscape module analysis.The expression levels of these key genes were subsequently extracted from the 38 breast cancer patients,and their correlations with clinical parameters and ultrasound features were systematically analyzed.Results Nine key genes were identified and classified into two subgroups:subgroup 1(MRPL12,MRPL13,MRPS12,MRPL14,NME3,HSPE1)and subgroup 2(DNA2,POLQ,RECQL4).Regarding ultrasound features,MRPS12 and MRPL14 expression was significantly upregulated in tumors with non-spiculated margins.In lesions exhibiting UMA-CPP>10%,MRPL12,MRPL13,MRPS12,and MRPL14 were significantly overexpressed,whereas DNA2 expression was downregulated.MRPL12 expression was notably elevated in cases with Alder grade III and perivascular distribution(P<0.05).Correlation analysis revealed that PI was positively correlated with MRPL13 and MRPS12,while AUC showed positive correlations with MRPL13 and HSPE1.AT was negatively correlated with MRPS12 and MRPL14,and TTP was negatively correlated with MRPS12.Regarding elasticity parameters,Emean was positively associated with MRPL12 and MRPS12;Emax with MRPL12 and NME3;Emin with MRPL12;and Esd with MRPL12,MRPL13,and MRPS12.Conversely,Esd demonstrated a negative correlation with DNA2(P<0.05).Notably,the majority of ultrasound features significantly associated with key genes were predominantly concentrated among four mitochondrial ribosomal protein genes MRPL12,MRPL13,MRPS12,and MRPL14 and exhibited consistent directional trends.Conclusion Mitochondrial oxidative stress-related gene expression correlates with specific ultrasound features of invasive ductal breast carcinoma,providing a non-invasive imaging basis for assessing mitochondrial oxidative stress within the tumor microenvironment.