水相稳定钙钛矿量子点生物探针制备及其在胱抑素C免疫层析中的应用OA
Preparation of Water-stable Perovskite Quantum Dot Bioprobes and Their Application in Cystatin C Lateral Flow Immunoassay
胱抑素C(Cystatin C,Cys C)是评估与监测肾功能的重要生物标志物,也是肾脏疾病早期筛查与居家监测的关键指标之一.免疫层析具有操作简便、检测快速等优势,适用于Cys C的定量检测;然而,传统信号标签的亮度与稳定性受限,制约了检测性能的进一步提升.为此,本研究构建了一种具备优异水相稳定性的钙钛矿量子点荧光生物探针用于Cys C的免疫层析检测.以介孔二氧化硅(Mesoporous silica nanoparticles,MSNs)为载体,在孔道内原位生成CsPbBr3量子点的同时构筑SiO2封闭层,得到CsPbBr3@MSNs@SiO2探针,其兼具较高荧光量子产率(PLQY≈93%)与良好水相稳定性(>120 h).基于该探针构建荧光免疫层析试纸条,实现了Cys C的快速定量检测,并表现出良好的特异性及浓度依赖的信号响应.本研究为肾功能相关标志物的居家与现场快速检测提供了一种亮度高、稳定性好的荧光标记策略,同时为金属卤化物钙钛矿量子点在生物分析与免疫层析检测中的应用拓展提供了新的思路与技术支撑.
Cystatin C(Cys C)is an important biomarker for evaluating and monitoring renal function and is widely used for early screening of kidney disease.Fluorescent lateral flow immunoassay is a convenient approach for rapid and quantitative Cys C testing,yet its analytical performance is often constrained by the brightness and stability of conven-tional signal labels.Here,we report a water-stable perovskite quantum dot(PQD)-based fluorescent bioprobe for use in Cys C LFIA.Mesoporous silica nanoparticles(MSNs)were employed as nanocarriers,enabling in situ formation of CsPbBr3 quantum dots within the mesopores while a SiO2 sealing layer was simultaneously constructed.The resulting CsPbBr3@MSNs@SiO2 probes exhibited high photoluminescence quantum yield(PLQY≈93%)and excellent water stability(>120 h),providing robust signal output for LFIA readout.Based on this bioprobe,a fluorescent LFIA strip was fabricated for quantitative Cys C detection,demonstrating good specificity and a clear concentration-dependent response.This work offers a bright and water-stable labeling strategy for point-of-care testing of renal function-related biomarkers and expands the utility of metal halide PQDs in bioanalysis and LFIA-based diagnostics.
陈志山;朱宏强;毛梓晴;林紫依;张绍安;徐佳;李战军;李杨
广州医科大学 生物医学工程学院,广东 广州 511436广州医科大学 生物医学工程学院,广东 广州 511436广州医科大学 生物医学工程学院,广东 广州 511436广州医科大学 生物医学工程学院,广东 广州 511436宁波大学 信息科学与工程学院,浙江 宁波 315000宁波大学 医学部,浙江 宁波 315000广州医科大学 生物医学工程学院,广东 广州 511436宁波大学 信息科学与工程学院,浙江 宁波 315000
数理科学
钙钛矿量子点胱抑素C免疫层析荧光即时检测
perovskite quantum dotsCystatin Clateral flow immunoassayfluorescencepoint-of-care testing
《发光学报》 2026 (3)
389-399,11
国家自然科学基金(52572173,52402192)广州市重点研发计划(2023B03J1239)宁波大学高级科学研究项目(GJPY2025010)Supported by National Natural Science Foundation of China(52572173,52402192)Guangzhou Key Research and Develop-ment Program(2023B03J1239)High-level Scientific Research Project of Ningbo University(GJPY2025010)
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