HS-SPME-GC-MS对不同品种大樱桃果实香气成分分析OA
Analysis of Aroma Components in Sweet Cherry Fruits of Different Varieties Using HS-SPME-GC-MS
探讨天水地区大樱桃香气成分差异,为甜樱桃优良品种的香味遗传育种、香气合成代谢、分子生物学研究方面提供理论依据.以天水地区主栽的7个大樱桃品种为试材,使用顶空固相微萃取气相色谱质谱联用技术(HS-SPME-GC-MS)鉴定果实中存在的香气物质,应用TIC峰面积归一法测定了各成分的相对含量.结果表明,7个大樱桃品种中共检出77种香气物质,其中布鲁克斯有24种、含香有25种、美早有16种、萨米脱有26种、拉宾斯有35种、斯坦勒有14种、红灯有12种.7个大樱桃品种果实香气物质含量各有差异,其中红灯醇类物质含量最高,为432.4g/kg;斯坦勒酯类物质含量最高,为2.26%;含香醛类物质含量最高,为488.8 g/kg;布鲁克斯酮类物质含量最高,为68.8 g/kg;其他类物质拉宾斯含量最高,为255.8 g/kg.7个大樱桃共有的物质为苯甲醛,被认定为关键香气成分;特有香气物质拉宾斯11种、布鲁克斯10种、萨米脱9种、含香7种、斯坦勒3种、美早2种.
This study investigated the differences in aroma components of sweet cherry in the Tianshui area,aiming to provide theoretical basis for aroma-related genetic breeding,aroma biosynthesis metabolism,and molecular biology research of superior sweet cherry variety.Using 7 main cultivated sweet cherry varieties in Tianshui area as test materials,aroma compounds present in the fruit were identified using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry(HS-SPME-GC-MS),and the relative content of each component was determined using the TIC peak area normalization method.Results showed that a to-tal of 77 compounds were detected in the 7 sweet cherry varieties,including 24 in Brooks,25 in Hanxiang,16 in Meizao,26 in Summit,35 in Lapins,14 in Stella,and 12 in Hongdeng.The Aroma compounds content in the fruit of the 7 sweet cherry varieties varied.Hong-deng variety had the highest content of alcohol substances at 432.4 g/kg,Stella variety had the highest content of esters at 2.26%,Hanx-iang variety had the highest content of aldehydes at 488.8 g/kg,Brooks variety had the highest content of ketones at 68.8 g/kg,and Lapins variety had the highest content of other substances at 255.8 g/kg.The substances shared by the 7 sweet cherries were benzaldehyde,which was identified as key aroma component.Unique aroma compounds were also identified,including 11 in Lapins,10 in Brooks,9 in Summit,7 in Hanxiang,3 in Stella,and 2 in Meizao.
周一帆;郭志刚;马新院;谭维军;陶淑芸;韩松凌
天水师范大学生物工程与技术学院,甘肃 天水 741000天水师范大学生物工程与技术学院,甘肃 天水 741000新疆农业大学园艺学院特色果树研究中心,新疆 乌鲁木齐 830052天水市果树研究所,甘肃 天水 741000天水市麦积区林业和草原局,甘肃 天水 741001天水师范大学生物工程与技术学院,甘肃 天水 741000
农业科技
大樱桃HS-SPME-GC-MS香气成分品种
Sweet cherryHS-SPME-GC-MSAroma componentVariety
《寒旱农业科学》 2026 (3)
245-254,10
天水市秦州区科技重大专项计划项目(2023-NCJKG-8373)中央引导地方科技发展资金项目(25ZYJE001)甘肃省重点研发计划—农业领域(24YFNE002)甘肃省科技计划—乡村振兴专项(24CXNE002)2026年现代寒旱特色农业科技支撑项目(KJZC-2026-18).
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