山葵内生细菌群落结构及其多样性OA

Wasabi as a high-value economic crop with both edible and medicinal uses,has stringent growth conditions and high cultivation difficulty,resulting in low yields that are insufficient to meet the growing market demand.Studying its endophytic bacteria can facilitate its cultivation and promote the development of biological resources derived from these endophytic bacteria.To investigate the community structure and diversity of endophytic bacteria in wasabi,high-throughput sequencing was performed on endophytic bac-teria in the roots,stems,and leaves of wasabi.The results showed that a total of 766 OTU were obtained from the roots,stems,and leaves of wasabi,among which 478,642,and 320 OTU were obtained from these three parts respectively;235 OTU were shared among these three parts.Species classification revealed that there were 35 phyla,85 classes,207 orders,358 families,and 766 genera of endophytic bacteria in wasa-bi.At the phylum level,the most dominant bacterial phylum for endophytic bacteria in wasabi stems and leaves is Proteobacteria,while the most dominant bacterial phylum for endophytic bacteria in wasabi roots was Actinobacteria.At the genus level,the most dominant bacterial genera for endophytic bacteria in roots,stems,and leaves of aquila were Lechevalieria,Staphylococcus,and Chryseobacterium respectively.The functional prediction of endophytic bacteria in wasabi indicated that they were mainly related to me-tabolism,environmental information processing,and genetic information processing.In summary,the com-munity structure of endophytic bacteria in different parts of wasabi varied to some extent,and the species had rich biodiversity.This study provided a theoretical basis for wasabi cultivation and the development of wasabi's endophytic bacterial biological resources.