基于转录组分析除草剂对羊肚菌的毒理机制OA

Taking the Morchella sextelata strain M6611 as the test material,the PDA plate culture method was adopted and combined with RNA-seq transcriptome analysis to study the effects of four commonly used herbicides(trifluralin,pendimethalin,S-metolachlor and acetochlor))on the mycelial growth of the mushroom,in order to provide references for analyzing the toxicological mechanism of herbicides on morel mushrooms.The results showed that all herbicides suppressed mycelial growth,with S-metolachlor exhibiting relatively weaker inhibition.Comprehensive transcriptome analysis revealed that herbicide treatment severely impaired critical energy metabolism pathways,particularly the processes of glycolysis/gluconeogenesis and the glyoxylate cycle.In conclusion,herbicides should be used cautiously in M.sextelata cultivation,and soil herbicide residue monitoring should be enhanced to reduce potential risks of mycelial growth inhibition and yield loss.