基于SSR标记与形态学的白掌F1代杂种真实性鉴定OA

Taking nine parental lines of Spathiphyllum kochii,eight hybrid F1 progenies,and a population of 39 hybrid F1 plants from the cross'Xingguang'×'Meijiu'as the test materials,polymorphic SSR marker analysis with morphological observation were combined to compare the efficiency of SSR markers and morphological methods in authenticating the hybridity of Spathiphyllum kochii F1 progenies,in order to establish an efficient and accurate early identification system to support Spathiphyllum kochii breeding.The results showed that eight pairs of SSR primers with paternal specific or complementary parental bands were selected from 16 polymorphic primers for hybrid identification.SSR amplification of the eight hybrid progenies all displayed paternal or biparental genotypes,achieving an authentication rate of 100％.Morphological observation clearly identified only six of the progenies('Qingmei''Xinghui''Weimei''Yangmeng 1''Yangmeng 2'and'Weimo')as true hybrids,while'Yushou'and'Mengxiang'could not be reliably distinguished based on morphology,giving a morphological identification efficiency of 75％.The efficiency of SSR markers was significantly higher than that of morphological methods,especially for true hybrids that did not exhibit obvious paternal traits.Further detection using the P14 marker on the 39 progenies from the'Xingguang'×'Meijiu'cross all produced paternal band patterns,with a true hybrid rate of 100％,reconfirming the high efficiency and reliability of SSR markers.In conclusion,SSR marker identification is accurate and efficient,and can serve as a key technique for early authenticity assessment of Anthurium hybrid progenies.