不同叶色紫苏叶抗炎作用比较及其与叶色值和化学成分的相关性分析OA
Comparison of Anti-inflammatory Effects of Perilla Folium with Different Leaf Colors and Its Correlation Analysis with Leaf Color Values and Chemical Compositions
目的:研究双紫紫苏叶(PP)、面绿背紫紫苏叶(GP)、双绿紫苏叶(GG)的抗炎作用,并分析抗炎作用与叶色色数值(L*、a*、b*、E*ab、R值、G值、B值)、紫苏醛、迷迭香酸、花青素的含量之间的关系.方法:采用CCK-8 法测定3 种紫苏叶对RAW 264.7 单核巨噬细胞存活率的影响,结合偏最小二乘判别分析法(PLS-DA)探讨不同叶色紫苏叶的差异.利用 Pearson 相关系数对红色像元分量均值(R值)、绿色像元分量均值(G值)、蓝色像元分量均值(B值)、红绿色值(a*值)、黄蓝色值(b*值)、亮度值(L*值)、总色值(E*ab值)等7 种叶色色数值、紫苏醛、花青素和迷迭香酸的含量及抗炎作用之间的关联性进行系统性分析,以揭示抗炎作用与色度特征及化学成分之间的内在联系.结果:PP、GP、GG均具有一定的抗炎作用,在 25 mg/mL 浓度下,PP 的存活率在 56％～94％,GP 的存活率在 49％～123％,GG的存活率在64％～110％.单因素方差分析表明,PP、GP、GG的抗炎作用无明显差异(P>0.05).PLS-DA结果显示,不同叶色紫苏叶各自归为一类,并筛选出紫苏醛、花青素、R 值、b*值、E*ab值、迷迭香酸和L*值等 7 个关键差异性成分.相关性分析显示,细胞存活率与b*值呈正相关.结论:三种叶色紫苏叶的抗炎作用无明显区别,紫苏叶叶色的b*值越高,其抗炎作用越强.
Objective:To study the anti-inflammatory effect of Perilla Folium with both surfaces purple(PP),Perilla Folium with upper surface green and the lower surface purple(GP),Perilla Folium with both surfaces green(GG)and to analyze the relationship between the anti-inflammatory effect,leaf color chromatic values(L,a*,b*,E*ab,R-value,G-value,and B-value),and the contents of perilla aldehydes,rosmarinic acid,and anthocyanins.Methods:The CCK-8 method was used to determine the effects of three types of Perilla Folium on RAW 264.7 monocyte-macrophage survival rates,and partial least squares discriminant analysis(PLS-DA)was used to explore the differences between perilla leaves with different leaf colors.Pearson correlation coefficients were used to systematically analyze the correlation between the seven leaf color values,including Red image component mean value(R-value),Green image component mean value(G-value),Blue image component mean value(B-value),Red green value(a*),Yellow blue value(b*),Brightness value(L*),and Total color value(E*ab),as well as the contents of perilla aldehyde,anthocyanin,and rosmarinic acid,and the anti-inflammatory effect in order to reveal the intrinsic links between anti-inflammatory effects and colorimetric characteristics and chemical composition.Results:PP,GP,and GG all showed certain anti-inflammatory effects.At a concentration of 25 mg/mL,the survival rates of PP,GP,and GG ranged from 56％to 94％,49％to 123％,and 64％to 110％,respectively.One-way ANOVA showed that there was no significant difference among the three.The results of PLS-DA showed that Perilla Folium with different leaf colors were each grouped into one category,and seven key differential indexes,including perilla aldehyde,anthocyanins,R value,b*,E*ab,rosemarinic acid and L*,were screened out.Correlation analysis showed that cell survival rates were positively correlated with b*-value.Conclusion:The anti-inflammatory effects of Perilla Folium did not significantly differ among the three leaf colors,and higher b*-value was associated with stronger anti-inflammatory effects.
刘雯丽;康帅;李灵犀;聂黎行;魏锋
沈阳药科大学功能食品与葡萄酒学院,辽宁 沈阳 117004中国食品药品检定研究院,北京 100050沈阳药科大学功能食品与葡萄酒学院,辽宁 沈阳 117004中国食品药品检定研究院,北京 100050中国食品药品检定研究院,北京 100050
医药卫生
紫苏叶抗炎作用色数值化学成分相关性
Perilla FoliumAnti-inflammatory effectColor valuesChemical compositionCorrelation
《中医药学报》 2026 (3)
34-38,5
国家重点研发计划中医药现代化专项(2023YFC3504100)中国食品药品检定研究院关键技术研究基金项目(GJJS-2022-10-2)
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