蚯蚓-秸秆联合修复设施盐渍化土壤的效果及其作用机制OA
Effect and mechanisms of remediation on salinized greenhouse soil by earthworm-straw
针对设施盐渍化土壤存在的生产障碍,本研究基于连作 10a的葡萄设施盐渍化土壤(电导率为2 301.55 μS·cm-1),引入蚯蚓和秸秆进行修复,设置不投放蚯蚓和秸秆(CK)、投放1%水稻秸秆(S1)、投放2%水稻秸秆(S2)、投放0.6 kg·m-2 威廉环毛蚓(E)、投放0.6 kg·m-2 威廉环毛蚓和1%水稻秸秆(S1E)、投放0.6 kg·m-2威廉环毛蚓和2%水稻秸秆(S2E)6 个处理,评估蚯蚓、秸秆联合对设施盐渍化土壤的修复效果,并探寻其中的关键微生物.试验90d时(试验结束),S1、S2、S1E、S2E处理的耕层(0～20 cm)土壤电导率较试验15d时分别下降46.43%、49.74%、52.34%和53.44%.与CK相比,S1E、S2E处理的土壤碱解氮、有效磷、速效钾、有机质和可溶性有机碳含量分别显著(p＜0.05)提升 12.68%～14.16%、12.45%～14.58%、14.89%～17.10%、19.48%～21.24%、30.19%～31.03%.三维荧光分析显示,土壤中的腐殖质类物质含量增加.添加秸秆的处理(S1、S2、S1E、S2E)中,鞘脂单胞菌属(Sphingomonas)、德沃斯氏菌属(Devo-sia)、交替赤杆菌属(Altererythrobacter)、Chthoniobacter、norank_f_Microscillaceae 等秸秆降解相关菌属,和假黄色单胞菌属(Pseudoxanthomonas)、苍黄杆菌属(Luteolibacter)、节杆菌属(Arthrobacter)、norank_f_norank_o_Gai-ellales等对盐分敏感的菌属的绝对丰度均较CK提高.总体来看,蚯蚓与秸秆联合修复通过促进土壤耕层盐离子向下迁移、增强土壤中秸秆的降解和对土壤微生物群落的调节,最终降低了设施盐渍化土壤的盐分,提升了土壤肥力,缓解了盐分对土壤微生物群落的胁迫.
Aimed at the production obstacles associated with salinized greenhouse soil,an experiment was conducted on soils with10 years of continuous cropping of grape(electrical conductivity of 2 301.55 μS·cm-1)to evaluate the remediation effect of the combined application of earthworms and straw on salinized greenhouse soil and identify the key functional microorganisms involved.Earthworms and rice straw were introduced for soil remediation,with six treatments established:no earthworms or straw addition(CK),1%rice straw addition(S1),2%rice straw addi-tion(S2),0.6 kg·m-2 Pheretima guillelmi addition(E),0.6 kg·m-2 P.guillelmi combined with 1%rice straw(S1E),and 0.6 kg·m-2 P.guillelmi combined with 2%rice straw(S2E).On the 90th day(the end of the experiment),the electrical conductivity of the topsoil(0-20 cm)in treatments S1,S2,S1E,and S2E decreased by 46.43%,49.74%,52.34%,and 53.44%,respectively,compared with that on the 15th day.Compared with CK,the contents of alkali-hydrolyzable nitrogen,available phosphorus,available potassium,organic matter,and dissolved organic carbon in S1E and S2E were significantly(p＜0.05)increased by 12.68%-14.16%,12.45%-14.58%,14.89%-17.10%,19.48%-21.24%,and 30.19%-31.03%,respectively.Besides,three-dimen-sional excitation-emission matrix(3D-EEM)fluorescence spectroscopy analysis revealed an increase in the content of humic substances in the soil.Among the treatments with straw addition(S1,S2,S1E,S2E),the absolute abun-dances of straw-degradation-related genera(e.g.,Sphingomonas,Devosia,Altererythrobacter,Chthoniobacter,norank_f_Microscillaceae)and salt-sensitive genera(e.g.,Pseudoxanthomonas,Luteolibacter,Arthrobacter,norank_f_norank_o_Gaiellales)were all higher than those in CK.Overall,the combined remediation with earthworms and straw reduced soil salinity,improved soil fertility,and alleviated the salt stress on soil microbial communities in salinized greenhouse soil,which was attributed to promoting the downward migration of salt ions in the topsoil,en-hancing straw degradation,and regulating the soil microbial community structure.
刘佳铭;张娟琴;王楠;马志梅;吕卫光;郑宪清;宋科;张翰林;张海韵;张月
上海应用技术大学 化学与环境工程学院,上海 201418||上海市农业科学院 生态环境保护研究所,上海 201403上海市农业科学院 生态环境保护研究所,上海 201403||上海市设施园艺技术重点实验室,上海 201403||上海市农业环境保护监测站,上海 201403上海上实现代农业开发有限公司,上海 202183上海市农业科学院 生态环境保护研究所,上海 201403上海市农业科学院 生态环境保护研究所,上海 201403||上海市设施园艺技术重点实验室,上海 201403||上海市农业环境保护监测站,上海 201403上海市农业科学院 生态环境保护研究所,上海 201403||农业农村部上海农业环境与耕地保育科学观测实验站,上海 201403||上海低碳农业工程技术研究中心,上海 201403上海市农业科学院 生态环境保护研究所,上海 201403||上海市农业环境保护监测站,上海 201403||上海低碳农业工程技术研究中心,上海 201403上海市农业科学院 生态环境保护研究所,上海 201403||上海市设施园艺技术重点实验室,上海 201403||上海市农业环境保护监测站,上海 201403上海市农业科学院 生态环境保护研究所,上海 201403||上海市设施园艺技术重点实验室,上海 201403||上海市农业环境保护监测站,上海 201403上海市农业科学院 生态环境保护研究所,上海 201403||上海市设施园艺技术重点实验室,上海 201403||上海市农业环境保护监测站,上海 201403
农业科技
盐渍化土壤秸秆蚯蚓土壤养分土壤微生物
salinized soilstrawearthwormsoil nutrientsoil microorganism
《浙江农业学报》 2026 (2)
327-338,12
上海市科技创新行动计划(22DZ1209404,23015820900)上海市农业科学院卓越团队建设计划(沪农科卓[2022]008)
评论