黑水虻蛹壳几丁寡糖的高效提取技术OA

To address the environmental concerns associated with traditional extraction methods for chitooligosacchar-ides from black soldier fly(Hermetia illucens)chrysalis shells,this study aimed to develop an environmentally friendly pretreatment and enzymatic hydrolysis process for the efficient conversion of these shells into chitooligosac-charides.Using black soldier fly chrysalis shells as the raw material,physical pretreatment was performed via ultra-fine grinding combined with ultrasonication.Organic acids,including formic acid,oxalic acid,and citric acid,were used for demineralization.Subsequently,enzymatic hydrolysis was conducted using various chitin-hydrolyzing en-zymes,such as keratinase,and the effects of temperature,enzyme dosage,and metal ion cofactors on hydrolysis effi-ciency were investigated.The feasibility of using acidic cellulase as a substitute for chitinase was also explored.The results showed that citric acid was most effective in removing calcium carbonate from the chrysalis shells;ultrasonica-tion did not significantly enhance subsequent enzymatic hydrolysis efficiency;chitinase hydrolysis efficiency increased with higher temperature and enzyme dosage;metal ions exhibited either promotive or inhibitory effects on the activity of different chitin-hydrolyzing enzymes;and acidic cellulase could effectively degrade chitin in the shells,performing comparably to chitinase.This study established a green extraction process for chitooligosaccharides from black soldier fly chrysalis shells based on organic acid demineralization and enzymatic degradation.It confirmed that acidic cellu-lase can serve as an effective alternative to chitinase,providing a feasible pretreatment strategy and enzyme selection basis for the high-value utilization of black soldier fly chrysalis shells.This contributes to enhancing the utilization ef-ficiency of black soldier fly biomass and promoting the circular development of agricultural organic waste.