以色列急性麻痹病毒感染对意大利蜜蜂分泌蜂王浆的影响OA

To investigate the impact of Israeli acute paralysis virus(IAPV)on royal jelly production in Apis mellifera ligustica,newly emerged worker bees were fed sucrose solutions containing three different concentrations of IAPV,following confirmation of the virus's preferential infection of the hypopharyngeal and mandibular glands.Transcrip-tome sequencing was first employed to preliminarily assess the virus's effects on royal jelly secretion.Subsequently,RT-qPCR and liquid chromatography were used to measure the relative expression levels of major royal jelly protein(mrjp)genes and the content of 10-hydroxy-2-decenoic acid(10-HDA),respectively.The results showed that high-concentration viral inoculation significantly reduced worker bee survival rates,with a median lifespan of only 16.5 days,and markedly disrupted protein and fatty acid synthesis pathways in the bees'heads.Further analysis by RT-qPCR and liquid chromatography revealed that,by day 18,high-concentration viral infection led to a 96%decrease in mrjp1 gene expression and an 11.98 μg reduction per bee in 10-HDA content.These findings demonstrate that IAPV impairs royal jelly secretion by suppressing the synthesis of major royal jelly proteins and fatty acids.