炮制前后的栀子-黄芩药对中5种活性成分含量及指纹图谱研究OA
Study on the contents of five active components and fingerprint of Gardenia jasminoides-Scutellaria baicalensis herb pairs before and after processing
目的 建立生栀子-黄芩药对和焦栀子-黄芩药对中5种活性成分含量的测定方法,并构建指纹图谱,探讨栀子炮制对药对中化学成分的影响.方法 采用高效液相色谱(HPLC)法检测10批生栀子-黄芩药对和10批焦栀子-黄芩药对中京尼平龙胆双糖苷、栀子苷、西红花苷Ⅰ、西红花苷Ⅱ、黄芩苷的含量;采用同一HPLC法,利用《中药色谱指纹图谱相似度评价系统(2012版)》建立10批生栀子-黄芩药对和10批焦栀子-黄芩药对的指纹图谱,并进行主成分分析和正交偏最小二乘-判别分析.结果 相较于生栀子-黄芩药对,焦栀子-黄芩药对中京尼平龙胆双糖苷的平均含量增加12.65%,栀子苷、西红花苷Ⅰ、西红花苷Ⅱ、黄芩苷的平均含量则分别下降7.86%、60.62%、62.07%、0.15%.10批生栀子-黄芩药对、10批焦栀子-黄芩药对的指纹图谱共有18个共有峰,相似度分别为0.997～1.000、0.988～1.000;指认了其中5个共有峰,分别为京尼平龙胆双糖苷(2号峰)、栀子苷(3号峰)、西红花苷Ⅰ(6号峰)、西红花苷Ⅱ(9号峰)、黄芩苷(10号峰).主成分分析和正交偏最小二乘-判别分析结果显示,生栀子-黄芩药对和焦栀子-黄芩药对可被明显区分,西红花苷Ⅰ、西红花苷Ⅱ的变量重要性投影值均大于1.结论 成功建立了测定栀子-黄芩药对中京尼平龙胆双糖苷等5种活性成分含量的方法,并绘制了指纹图谱.炮制可使药对中京尼平龙胆双糖苷的含量增加,栀子苷、西红花苷Ⅰ、西红花苷Ⅱ、黄芩苷的含量降低;西红花苷Ⅰ、西红花苷Ⅱ可作为药对质量控制的潜在标志物.
OBJECTIVE To establish the method for determining the contents of five active components in raw and charred herb pairs of Gardenia jasminoides-Scutellaria baicalensis,construct their fingerprints,and investigate the effects of G.jasminoides processing on chemical constituents in the herb pairs.METHODS The HPLC method was used to determine the contents of genipin 1-β-D-gentiobioside,geniposide,crocin Ⅰ,crocin Ⅱ and baicalin in ten batches of raw G.jasminoides-S.baicalensis herb pairs and ten batches of charred G.jasminoides-S.baicalensis herb pairs.Using the same HPLC method,chromatographic fingerprints for the ten batches of raw herb pairs and ten batches of processed herb pairs were established with the Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine(2012 edition).Principal component analysis and orthogonal partial least squares-discriminant analysis were performed.RESULTS Compared with the raw G.jasminoides-S.baicalensis herb pair,the average content of genipin 1-β-D-gentiobioside in the charred G.jasminoides-S.baicalensis herb pair increased by 12.65%;while the average contents of geniposide,crocin Ⅰ,crocin Ⅱ and baicalin decreased by 7.86%,60.62%,62.07%and 0.15%,respectively.Chromatographic fingerprints of ten batches of raw herb pairs and ten batches of processed herb pairs shared 18 common peaks with similarities ranging from 0.997 to 1.000 and 0.988 to 1.000,respectively.Five common peaks were identified:genipin 1-β-D-gentiobioside(peak 2),geniposide(peak 3),crocin Ⅰ(peak 6),crocin Ⅱ(peak 9)and baicalin(peak 10).Principal component analysis and orthogonal partial least squares-discriminant analysis results showed that the raw G.jasminoides-S.baicalensis herb pair and the charred G.jasminoides-S.baicalensis herb pair could be clearly distinguished.Variable importance in the projection(VIP)values for crocin Ⅰ and crocin Ⅱ were both greater than one.CONCLUSIONS A method has been successfully developed for the determination of five active components,including genipin 1-β-D-gentiobioside,in the G.jasminoides-S.baicalensis herb pair,and its fingerprint has been drawn.Processing is found to increase the content of genipin 1-β-D-gentiobioside,while decreasing the levels of geniposide,crocin Ⅰ,crocin Ⅱ and baicalin in the herb pair.Furthermore,crocin Ⅰ and crocin Ⅱ could serve as potential quality markers for the quality control of this herb pair.
李玉;祁俊;吴萍
芜湖市中医医院中药炮制室,安徽 芜湖 241000芜湖市中医医院中药炮制室,安徽 芜湖 241000芜湖市中医医院药剂科,安徽 芜湖 241000
医药卫生
炮制生栀子焦栀子黄芩药对含量测定指纹图谱
processingraw Gardenia jasminoidescharred G.jasminoidesScutellaria baicalensisherb paircontent determinationfingerprint
《中国药房》 2026 (6)
727-732,6
全国老药工传承工作室建设项目(No.国中医药人教函[2024]255号)
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