lncRNA LOC101928477在食管鳞状细胞癌中的作用研究OA

Objective To investigate the biological effects of long non-coding RNA(lncRNA)LOC101928477 on proliferation,migration,apoptosis and autophagy of human esophageal squamous cell carcinoma(ESCC)ECA109 cells,and to explore the associated molecular regulatory mechanisms.Methods ECA109 cells were divided into five groups:Group A(blank control,ECA109 cells untreated),Group B(empty vector control,ECA109 cells treated with oe-NC),Group C(LOC101928477 overexpression,ECA109 cells treated with oe-LOC101928477),Group D(miR-139-5p inhibition,ECA109 cells treated with miR-139-5p inhibitor),and Group E(combined intervention,ECA109 cells treated with both miR-139-5p inhibitor and oe-LOC101928477).Cell viability was detected by the Cell Counting Kit-8(CCK-8)assay.Cell apoptosis was analyzed by flow cytometry.Cell migration ability was assessed by the scratch assay.The mRNA expressions of LOC101928477 and miR-139-5p were detected by quantitative real-time polymerase chain reaction(qRT-PCR).The expressions of autophagy-related proteins(Beclin1,LC3-Ⅱ/LC3-I,P62)were detected by Western Blotting.The levels of tumor necrosis factor-α (TNF-α)and interleukin-6(IL-6)were determined by enzyme-linked immunosorbent assay(ELISA).Results Compared with Group A,there were no significant differences in all detected indicators in Group B(P＞0.05).Compared with Group B,the cell viability and migration ability of ECA109 cells in Group C were significantly decreased,the apoptosis rate was significantly elevated,the expression of Beclin1 and the ratio of LC3-Ⅱ/LC3-I were remarkably upregulated,the protein expression of P62 was prominently downregulated,and the levels of TNF-α and IL-6 were significantly increased(all P＜0.05).Compared with Group C,LOC101928477-induced phenotypic effects and autophagy activation as mentioned above were significantly reversed in Group D(all P＜0.05).Compared with Group D,the cell viability and migration ability in Group E were further decreased,accompanied by further increases in the apoptosis rate,autophagic activity,and levels of TNF-α and IL-6(all P＜0.05).Conclusion The long non-coding RNA LOC 101928477 activates autophagy in human esophageal squamous cell carcinoma ECA109 cells,inhibits cell proliferation and migration,promotes apoptosis,and upregulates the levels of inflammatory cytokines TNF-α and IL-6.The tumor-suppressive effects of LOC101928477 may be mediated through regulation of miR-139-5p expression.These findings provide a novel theoretical basis for targeted therapy in esophageal squamous cell carcinoma.