非洲猪瘟病毒B169L蛋白多克隆抗体制备OA

B169L is a membrane protein encoded by the African swine fever virus(ASFV),and its biological function remains unclear.To facilitate the investigation of its potential roles,a polyclonal antibody against B169L was generated in this study.Bioinformatic analysis predicted that B169L contained two transmembrane domains and a signal peptide,suggesting its localization in the endoplasmic reticulum.The truncated B169L protein lacking transmembrane regions was expressed and purified using an Escheri-chia coli prokaryotic expression system,and used to immunize rabbits for polyclonal antibodies production.Western-blot analysis demonstrated that the obtained antibodies specifically recognized the B169L protein expressed in ASFV-infected cells.Collectively,these results indicated that a specific polyclonal antibody against B169L was successfully prepared,providing an essential tool for future studies on the structure and function of the ASFV membrane protein B169L.