非洲猪瘟病毒p30、p54和p72蛋白多表位抗原的制备及其免疫原性评价OA

This study aimed to prepare multi-epitope antigens of African swine fever virus(ASFV)and evaluate its application in vaccine.We designed a prokaryotic recombinant plasmid expressing the molecular chaperone TF16 and ASFV multi-epitope antigen,which can solubly express ASFV-pBT multi-epi-tope recombinant protein.After purification and identification,mice were immunized and their immuno-genicity was evaluated,providing a test basis for the research of subunit vaccines against African swine fever.The results showed that ASFV-pBT can be solubly expressed in Escherichia coli and specifi-cally recognized by ASFV antibody positive serum.The ASFV-pBT immunized group achieved serum antibody titers up to 1∶32 000,and ASFV-pBT could effectively activate the proliferation of T lymphocytes in mice.Compared with the negative control group,mice in the ASFV-pBT group exhibited significantly in-creased proportions of CD4+and CD8+T lymphocytes(P＜0.001)and IFN-γ and IL-4 cytokine levels were markedly elevated(P＜0.001).In vivo and in vi tro double validation showed that ASFV-pBT recombinant protein possessed strong immunogenicity,inducing robust humoral and cellular immune respons es,thereby laying reference for ASFV epitope vaccine development.