高效液相色谱法测定芝麻油中芝麻素的含量OA
Determination of Sesamin Content in Sesame Oil by High-Performance Liquid Chromatography(HPLC)
目的:建立高效液相色谱法(HPLC)准确,快速测定芝麻油中芝麻素含量的方法.方法:采用XSelect CSH C18色谱柱(4.6 mm×250 mm,5 μm);流动相为甲醇-水(体积比 80∶20,V∶V);流速0.8 mL/min;检测波长287 nm;柱温35℃;进样量10 μL.结果:芝麻素的线性回归方程为Y=0.000 08X-1.437 9(Y为峰面积,X为浓度,μg/mL),相关系数R2=0.995 1;线性范围为4.987 008 μg/mL～149.702 4 μg/mL;加样回收率试验显示,平均回收率为101.03%,方法精密度良好.结论:此方法操作简便,灵敏度高,重复性好,能准确测定芝麻油中芝麻素的含量,适用于芝麻油质量控制及相关分析.
[Objective]To establish a high-performance liquid chromatography(HPLC)method for accurate and rapid determination of sesamin content in sesame oil.[Method]The analysis was performed using an XSelect CSH C18 chromatographic column(4.6 mm×250 mm,5 μm).The mobile phase consisted of methanol-water(volume ratio 80:20,V:V).at a flow rate of 0.8 mL/min.The detection wavelength was set at 287 nm,the column temperature was maintained at 35 ℃,and the injection volume was 10 μL.[Result]The linear regression equation for sesamin was Y=0.000 08X-1.437 9(Y:peak area;X:concentration,μg/mL),with a correlation coefficient(R²=0.995 1).The linear range was 4.987 008 μg/mL to 149.702 4 μg/mL.The spike recovery test showed an average recovery rate of 101.03%,indicating good method precision.[Conclusion]This method is simple to operate,highly sensitive,and exhibits good repeatability.It can accurately determine the sesamin content in sesame oil,making it suitable for quality control and related analyses of sesame oil.
洋西吉;吴宗耀;占堆
西藏藏医药大学,西藏 拉萨 850000西藏藏医药大学藏医药与高原生物重点实验室,西藏 拉萨 850000西藏藏医药大学,西藏 拉萨 850000
高效液相色谱法芝麻油芝麻素
high performance liquid chromatography(HPLC)sesame oilsesamin
《中国食物与营养》 2026 (3)
15-20,6
2024年度藏医药标准化研究项目(西藏地方藏医特色食材目录编制研究,项目编号:ZYBZ2025002).
评论