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基于SSR的横断山古核桃遗传多样性评价OA

Genetic Diversity of Ancient Walnut Resources in the Hengduan Mountains Using SSR Markers

中文摘要英文摘要

我国西南部横断山区分布有丰富的核桃(Juglans regia)、泡核桃(J.sigillata)古树资源,本研究使用11对SSR引物分析了8个核桃与泡核桃古树群体共159个个体的遗传变异状况.分析结果显示,总群体的平均等位基因数7.727、有效等位基因数2.234、期望杂合度0.496、观察杂合度0.461,表明总群体存在中等遗传多样性,且泡核桃群体遗传多样性高于核桃群体.同时,泡核桃群体的遗传分化系数高于核桃群体,且群体间的遗传变异均占有一定比重.UPGMA聚类与遗传结构分析表明,与采样群体分为核桃与泡核桃2个种的情况一致,8个群体可明显分为核桃与泡核桃2个类群,核桃与泡核桃分别又分为3和2个类群,具有一定程度的地理分布特征,但是Mantel检测结果表明,这种分布特征与地理距离并没有显著的相关关系.

Abundant ancient walnut resources are distributed in Hengduan Mountains of southwest China.In this study,11 pairs of SSR primers were used to detect population genetic diversity of 159 trees sampled from 8 populations of Juglans regia and J.sigillata.The results revealed that the total population has an average value of number of 7.727 alleles,2.234 effective alleles,an expected heterozygosity of 0.496,and an observed heterozygosity of 0.461,indicating medium genetic diversity,and that the genetic diversity of J.sigillata population was higher than that of J.regia population.Meanwhile,the genetic differentiation coefficient of J.sigillata population was higher than that of J.regia population,and the genetic variation among the populations accounted for a certain proportion.UPGMA clustering and the STRUCTURE anal-ysis showed that the 8 populations could be clearly divided into 2 groups of J.regia and J.sigillata re-spectively,consistent with the actual situation.J.regia and J.sigillata populations were further divided into 3 groups and 2 groups,respectively,with a certain degree of geographical distribution characteristics,but Mantel's detection showed that the distribution characteristics were not significantly correlated with geographical distance.

吴万波;王丽;唐欢;王泽亮

四川省林业科学研究院,四川 成都 610081黑水县林业和草原局,四川 黑水 623500木里藏族自治县林业和草原局,四川 木里 615800四川省林业科学研究院,四川 成都 610081

农业科技

古树资源核桃泡核桃横断山区遗传多样性

ancient walnut resourcesJ.regiaJ.sigillataHengduan Mountainsgenetic diversity

《西北林学院学报》 2026 (2)

31-39,9

四川省省级科研院所基本科研业务费项目(2024JBKY02).

10.3969/j.issn.1001-7461.2026.02.04

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