源自绿萝叶提取物的红光碳点制备和生物成像应用OA

Carbon dots(CDs)have been widely applied in fluorescence imaging both in vitro and in vivo.However,key challenges remain to be addressed,including the poor specificity of CDs as biological markers and their relatively low fluorescence quantum yield(QY)in the red emission region.In this study,we synthesized red fluorescent carbon dots(designated as EA-CDs,λex/λem=400 nm/660 nm)using a natural plant-derived precursor ethanol extract from Epipremnum aureum leaves via a one-pot solvothermal method.The EA-CDs exhibit a small particle size(average diameter:3.9 nm),high fluorescence QY(15.4%in ethanol at λem=660 nm),low toxicity(both in vitro and in vivo),and favorable lipophilicity(oil-water partition coefficient LogP>0),making them suitable for biological fluorescence imaging and labeling applications.Experimental results indicate that these red-emitting CDs can not only effectively label plant cell membranes,but also serve as an intestinal fluorescence imaging probe in zebrafish models.This suggests their potential as a universal red-emissive bio-membrane dye with high QY.Furthermore,this work pioneers a novel application approach for ornamental plants like Epipremnum aureum.