肝素结合蛋白快速检测方法的建立OA

Sepsis is a systemic inflammatory response syndrome caused by infection.It is often complicated by organ dysfunction,which is a major contributor to mortality of patients with severe infections.Heparin-binding protein(HBP),a multifunctional mediator derived from neutrophils,exhibits levels closely correlated with the severity of sepsis and has great potential as a biomarker for early diagnosis and prognosis of sepsis.Herein,after vector construction and prokaryotic expression,the purified recombinant HBP was used as an immunogen to immunize mice.Following this,four stable cell lines secreting anti-HBP monoclonal antibodies were selected through cell fusion technology.The properties of the antibodies were analyzed by indirect en-zyme-linked immunosorbent assay(ELISA).Finally,a colloidal gold immunochromatographic assay for HBP was established using mAb 11 as the labeling antibody and mAb 18 as the capture antibody.This study suc-cessfully generated monoclonal antibodies against HBP using the recombinant HBP,and preliminarily estab-lished a rapid detection method for HBP.This method is of significant importance in resource-limited areas or scenarios where rapid assessment of infection severity is required.