姜黄素复合GelMA水凝胶抑制兔耳增生性瘢痕的形成OA

To investigate the inhibitory effect of early intervention with curcumin(Cur)-loaded GelMA(Cur/GelMA)hydrogel on hypertrophic scarring,a 25 μmnol/L Cur/GelMA hydrogel was prepared.Its internal structure and mean pore size were characterized by scanning electron microscope(SEM),and the release ki-netics of curcumin were evaluated by drug release assays.Biocompatibility of the Cur/GelMA hydrogel was assessed by CCK-8 assay and live/dead staining.Reverse transcription quantitative PCR(RT-qPCR)was employed to analyze the effects of Cur/GelMA on the gene expression of transforming growth factor-β1(TGF-β1),Smad2,and Smad3 in hypertrophic scar fibroblasts(HSFBs).Then Cur/GelMA hydrogel was ap-plied to the hypertrophic scars for early intervention in an established rabbit ear scar model.Gross observa-tion and hematoxylin-eosin(HE)staining were performed at 7,14,and 28 days after treatment to evaluate wound healing and scar formation,and immunofluorescence and immunohistochemical staining were con-ducted at 28 days to assess expression levels of platelet-endothelial cell adhesion molecule(CD31)and α-smooth muscle actin(α-SMA).The results showed that the prepared Cur/GelMA hydrogel exhibited a porous structure with appropriate pore size,an adequate sustained-release profile for curcumin,and good biocom-patibility.Cur/GelMA significantly reduced TGF-β1,Smad2,and Smad3 gene expression in HSFBs,markedly improved the color,texture,smoothness,and healing rate of regenerated skin in the rabbit ear scar model,inhibited fibroblast proliferation and excessive collagen deposition,and promoted relatively organized colla-gen fiber arrangement.Expression levels of CD31 and α-SMA were significantly decreased.In summary,early application of Cur/GelMA hydrogel effectively attenuates hypertrophic scar formation in rabbit ears,likely via inhibition of fibroblast hyperproliferation and neovascularization,as well as promotion of myofi-broblast apoptosis.