山慈菇基原植物叶绿体基因组特征及其比较研究OA

Objective To establish a robust molecular foundation for accurate seedling identification,this study addresses the limitations of traditional morphological methods for identifying seedlings of Cremastrae Pseudobulbus Pleiones Pseudobulbus(CPPP)and explores the application of high-resolution molecular markers.Methods The chloroplast genomes of three species of CPPP were sequenced,assembled,and annotated.Subsequently,comparative whole-genome analyses were conducted to detect sequence variation loci.A maximum likelihood phylogenetic tree was constructed to assess the discriminatory power,and specific primers were designed based on hypervariable regions.Results The chloroplast genomes of the three species exhibited a typical circular quadripartite structure,with lengths ranging from 158456 to 158612 bp,and a total of 131 to 136 genes were annotated.Simple sequence repeat(SSR)loci were abundant and primarily consisting of A/T mononucleotide repeats.Codon usage exhibited a significant preference for A/U terminal bases.Phylogenetic analysis revealed that the genus Cremastra and Pleione formed a highly supported monophyletic group.Genome variation analysis identified the coding regions rps16,atpF,psbH,as well as intergenic regions accD-psaI,atpB-rbcL,and psbM-trnD-GUC,as highly variable.Conclusion Chloroplast genomes provide an efficient and accurate method for identifying the original plants of CPPP.The six pairs of specific primers developed in this study offer candidate tools for molecular identification.This research has laid a molecular foundation for the establishment of the ginseng seedling identification system,and is of great significance for standardizing the medicinal material market and ensuring the quality and safety of traditional Chinese medicine.