不同产地晾制后雪茄烟叶的代谢组差异分析OA

To clarify the impact of different production areas on the metabolic characteristics and agronomic traits of cigar tobacco,in this study,the Shifang area in Sichuan and the Linqu area in Shandong were taken as the research objects,five cigar tobacco lines(QX102,QX105,QX107,QX200,and QX205)with the same genetic background were selected.The non-targeted derivatization GC-MS metabolomics technology combined with the orthogonal partial least squares discriminant analysis(OPLS-DA)model was used to systematically analyze the differences in the metabolome of tobacco leaves in the two places and the key metabolic pathways.The results showed that a total of 192 metabolites were identified from tobacco leaves,among which 44 showed significant regional specificity of the production areas.The accumulation amounts of uracil,tyramine,and trimethylsilyl phosphate in the Sichuan production area were 17.59 times,14.22 times,and 5 times higher than those in Shandong,respectively.The differential metabolites were mainly enriched in the amino acid metabolism and carbon-nitrogen balance pathways.The analysis of agronomic traits showed that the plant height 221.01 cm,stem circumference 8.59 cm,and the number of leaves of tobacco in the Shandong production area were significantly better than those in Sichuan,and the population phenotypic stability was higher.Its metabolic regulation network was closely related to the upregulated expression of key genes in nitrogen metabolism.The QX105 line showed metabolomic specificity in both places:uracil accumulated abnormally in QX105 planted in Sichuan,while the monoterpene synthase activity was relatively high in QX105 planted in Shandong,driving the synthesis of terpenoids such as phytol,highlighting the regulatory potential of the interaction between genotype and environment on the metabolic network.In conclusion,the synergistic response mechanism of metabolite accumulation and agronomic traits of cigar tobacco under different ecological environments was clarified,it was confirmed that the Shandong production area was more conducive to the formation of high-yield and high-quality tobacco leaves,the feasibility of metabolomics-assisted variety adaptability evaluation was verified,and it was suggested that the planting scale of cigar tobacco should be expanded with Linqu in Shandong as the central demonstration area and promoting of high adaptability product lines such as QX105 should be prioritized.