基于ISSR分子标记的山药种质资源遗传多样性分析OA

To clarify the genetic diversity of yam(Dioscorea opposita Thunb.)germplasm resources and guide variety selection,in this study,the genetic diversity(including Nei's gene diversity index H,Shannon information index I,and effective allele number Ne)of 34 yam germplasms was analyzed using Inter Simple Sequence Repeat(ISSR)molecular marker technology.Further,population structure analysis,cluster analysis,and principal component analysis were conducted.The results showed that a total of 109 polymorphic bands were detected in 34 yam materials,with a polymorphism rate of 95.94%,effective allele number was 1.430 5,Nei's gene diversity index was 0.267 0,and Shannon index was 0.417 3.These results indicated that the yam materials had a high level of molecular polymorphism.Population structure analysis revealed that there were 4 gene pools among the 34 materials,with gene pool 1 and 2 being the dominant sources,accounting for 47.06%and 29.41%respectively.The results of cluster analysis revealed that the genetic similarity coefficients of 34 yam materials ranged from 0.54 to 0.91,and when GS was 0.64,34 yam germplasms were clustered into four major groups.Principal component analysis revealed that the 34 yam materials were divided into four independent groups with a relatively dispersed population distribution and no obvious overlap among different groups.This indicated a clear genetic structure of the tested materials and significant genetic differences between populations.