荔枝LcGAMYB基因家族鉴定及矮化相关分子标记开发OA

Dwarfism is a key trait for Litchi chinensis breeding and cultivation.Focusing on the candidate gene LcGAMYB23 implicated in dwarfism by prior quantitative trait locus(QTL)mapping,we evaluated the MYB transcription factor family background and its molecular application potential.A genome-wide bioinformatic survey identified 151 members of the LcMYB gene family.Conserved motif and domain analyses showed that all family members harbored the canonical Myb_DNA-binding domain,indicating high sequence conservation in litchi.We further cloned and sequenced LcGAMYB23 from three standard(non-dwarf)cultivars(9918,Feizixiao,FZX;Sanyuehong,SYH)and three dwarf culti-vars(Nuomici,NMC;Yamulong,YML;Ziniangxi,ZNX),and detected consistent sequence differences between the two groups.Based on the divergent single-nucleotide polymorphism(SNP)sites,we developed molecular markers and vali-dated them across 70 cultivars.The markers effectively discriminated standard versus dwarf types at the cDNA level,with concordant results at the genomic DNA level.The findings would provide practical tools for molecular typing and marker-assisted selection of dwarfism in litchi and lay the groundwork for functional elucidation of LcGAMYB23.