基于广泛靶向代谢组学分析不同生长发育时期莲雾果皮代谢物的差异OA

The widely targeted metabolisms of fruit pericarp was determined,the differential metabolites were screened and the metabolic pathway was analyzed to understand the metabolomics difference of fruit pericarp at its different growth and development stages in wax apple(Syzygium samarangense).The samples were picked at 15 d(S1),30 d(S2),45 d(S3)and 60 d(S4)after flower respectively.A total of 946 metabolites were detected from wax apple fruit pericarp,including 177 amino acids and their derivatives,140 flavonoids,96 sugars and their derivatives,96 organic acids and their derivatives,89 lipids,65 nucleotides and their derivatives,and 283 other metabolites.There were 65,88,59,88,112,and 103 differential metabolites in S1 vs S2,S2 vs S3,S3 vs S4,S1 vs S3,S1 vs S4,and S2 vs S4 respectively.There were only 6 common differential metabolites in six groups,of which 5 metabolites belonged to amino acids and deriva-tives and 1 belonged to flavonoid.The differential metabolites of fruit pericarp at different growth and development stages mainly included amino acids and their derivatives,flavonoids,sugars and their derivatives,lipids.The number of differential metabolites of flavonoids,sugars and their derivatives decreased first and then increased,while the number of lipid metabolites increased first and then decreased with the growth and development of fruit.Most flavonoid me-tabolites were down-regulated,while sugars and their derivatives were up-regulated.There were more lipid differential metabolites in S2 vs S3,and only 1 in S3 vs S4.The most significant enrichment pathway of differential metabolites of wax apple fruit pericarp were biosynthesis of starch and sucrose,phenylalanine,D-amino acid,galactose,terpenoids and ster-oids.This study would provide theoretical support and data references for the regulation of fruit quality and harvest period of wax apple.