橡胶园黄精套种与玫瑰育苗对土壤细菌群落的效应探究OA

This study aimed to explore the effects of interplanting Polygonatum sibiricum and raising rose seedlings on soil bacterial communities in rubber plantations and to provide a scientific basis for the high-yield cultivation of P.sibiricum,effi-cient seedling raising of roses,and the development of related industries under rubber forests.The bacterial community struc-ture and diversity of noninterplanted soil,P.sibiricum-interplanted soil,and rose seedling-raising soil in rubber plantations of Puwen Town,Jinghong City,were compared and analyzed by 16S rRNA gene sequencing and bioinformatics analysis.For the P.sibiricum samples,the abundance and diversity of bacterial communities in the rhizosphere soil sample(HJ1)were sig-nificantly higher than those in the root zone soil(HJ2)and noninterplanted soil(HJ3).It could be inferred that root exudates of P.sibiricum could promote the growth and reproduction of certain bacterial communities.However,the abundances of Gemmatimonadetes,Nitrospirota,and Cyanobacteria were extremely low in HJ1,which might be due to the inhibition of these three phyla by P.sibiricum root exudates.For the rose samples,the soil bacterial community in the rose seedling-raising soil(MG3)had the highest abundance and diversity compared with the rhizosphere soil(MG1)and root zone soil(MG2).It could be inferred that root exudates of roses could inhibit the growth and reproduction of certain bacterial communities,espe-cially Proteobacteria;the abundances of Fusobacteriota and Crenarchaeota were extremely low in MG1.LEfSe analysis re-vealed that all six groups of soil samples had distinguishable biomarkers.Interplanting P.sibiricum and raising rose seedlings in rubber plantations had significant effects on the structure and diversity of soil bacterial communities under rubber forests.The abundance and diversity of soil bacteria in P.sibiricum-interplanted soil were significantly higher than those in noninter-planted soil.In contrast,the abundance and diversity of soil bacteria in rose seedling-raising soil were significantly lower than those in nonseedling-raising soil.This study deepened the understanding of the effects of P.sibiricum interplanting and rose seed-ling raising on soil microorganisms in rubber forests,obtained preliminary conclusions,provided scientific references for the develop-ment of an underrubber forest economy,and laid a scientific foundation for the diversified development of this industry.