基于蒲公英来源纳米纤维素的载氧凝胶制备与细胞活性研究OA

OBJECTIVE To prepare an oxygen-loaded gel based on Taraxaci Herba-derived cellulose nanofibrils and investi‑gate its activity in promoting cell proliferation,migration,and angiogenesis.METHODS Three types of oxidized cellulose nanofibers(CNF--1,CNF--2,CNF--3)with different degrees of substitution were synthesized via the TEMPO-mediated oxidation method.The metal ion species was determined by evaluating the cytotoxicity,cell proliferation-promoting effects,and gel-forming ability with oxi‑dized cellulose nanofibrils.The optimal preparation conditions for the oxidized cellulose nanofibril gel were established,and oxygen nanobubbles were incorporated into the gel to assess its cellular activity.RESULTS CNF--3 contained the most carboxyl groups and exhibited the fastest gelation rate.As a concentration of 2.0%,it can quickly form a stable gel.Zn2+demonstrated low cytotoxicity,strong proliferative activity,and favorable gel-forming performance.The CNF--3 gel crosslinked with Zn²⁺ effectively encapsulated oxygen nanobubbles and exhibited favorable activity in promoting cell proliferation,migration,and angiogenesis.CONCLUSION Ta-raxaci Herba-derived cellulose nanofibers can be crosslinked into a gel via metal ions and serve as a carrier for oxygen nanobubbles,showing potential for applications in wound healing and related treatments.