基于GC-MS探究不同药性人参类中药对元气虚大鼠短链脂肪酸的影响OA

Objective This study aimed to determine the content of short-chain fatty acids(SCFAs)in the feces of rats with primordial Qi deficiency using gas chromatography-mass spectrometry(GC-MS)and to investigate the effects of ginseng-based traditional Chinese medicines with different medicinal properties on SCFA levels in rats with primordial Qi deficiency.Methods SPF-grade SD male rats were randomly divided into groups.The control group was subcutaneously injected with normal saline,while the remaining rats were used to establish a model of primordial Qi deficiency by subcutaneous injection of isoproterenol(ISO,50 mg/kg)combined with dietary restriction.In the low-dose administration experiment,the groups included:control group,model group,ginseng administration group,red ginseng administration group,black ginseng administration group,ginseng leaf administration group,American ginseng administration group,dried ginger administration group,and aconite administration group.In the high-dose administration experiment,the groups included:control group,model group,ginseng administration group,red ginseng administration group,black ginseng administration group,ginseng leaf administration group,American ginseng administration group,phellodendron bark administration group,gypsum administration group,and captopril administration group.GC-MS was used to measure the concentrations of caproic acid,isovaleric acid,valeric acid,isobutyric acid,butyric acid,propionic acid,and acetic acid in fecal samples from each group.Results Compared with the control group,the fecal SCFA levels in the model group were significantly reduced,reflecting the trend of SCFA changes in the primordial Qi deficiency model.In the low-dose experiment,the content of acetic acid in red ginseng was higher than the model group(P<0.05,P=0.235),the content of acetic acid in black ginseng was higher than the model group(P<0.05,P=0.016),the content of propionic acid in the American ginseng was higher than the model group(P<0.05,P=0.015),the content of isobutyric acid in the American ginseng was higher than the model group(P<0.05,P=0.019),the content of isovaleric acid in the American ginseng was higher than the model group(P<0.01,P=0.001),the content of valeric acid in the American ginseng was higher than the model group(P<0.01,P=0.006),and the content of heptanoic acid in the American ginseng was higher than the model group(P<0.01,P=0.001).The content of heptanoic acid in ginseng leaf extract was higher than the model group(P<0.01,P=0.003).In the high-dose experiment,the content of propionic acid in the American ginseng was higher than the model group(P<0.01,P=0.008),the content of propionic acid in the American ginseng was higher than the model group(P<0.05,P=0.030),the content of isobutyric acid in red ginseng was higher than the model group(P<0.05,P=0.030),the content of isobutyric acid in ginseng leaf extract was higher than the model group(P<0.01,P=0.004),the content of butyric acid in red ginseng was higher than the model group(P<0.01,P=0.000),the content of butyric acid in black ginseng was higher than the model group(P<0.01,P=0.001),the content of valeric acid in ginseng leaf extract was higher than the model group(P<0.01,P=0.002),and the content of valeric acid in the American ginseng was higher than the model group(P<0.05,P=0.026).Conclusion Ginseng-based traditional Chinese medicines with warm or hot medicinal properties,at both low and high doses,primarily regulated acetic acid and butyric acid.In contrast,ginseng-based traditional Chinese medicines with cold or cool medicinal properties,at both low and high doses,mainly regulated propionic acid,isobutyric acid,isovaleric acid,valeric acid,and acetic acid.