miR-486-5p过表达的脐带间充质干细胞通过抑制铁死亡减轻放射性心脏损伤OA

Objective To evaluate the protective effect of miR-486-5p modified umbilical cord mesenchymal stem cells(UCMSCmiR-486)against radiation-induced heart damage(RIHD)and explore the mechanism.Methods 20 Gy of 60Co g-ray was used to establish a model for radiation-induced cardiomyocytes and cardiac damage to mice before UCMSCmiR-486(1×106 cells per mouse)was treated after radiation.Cell proliferation was detected using CCK-8 kit 48 hours post-treatment.Apoptosis,changes of mitochondrial membrane potential,and the opening of the mitochondrial permeability transition pore of cardiomyocytes were detected by flow cytometry.Small animal ultrasound was used to detect and analyze the cardiac function of mice 42 days later.Changes of cardiac histopathology were detected via HE staining and Masson staining.The ultrastructure of myocardia was observed under a transmission electron microscope(TEM).Real-time quantitative PCR(RT-qPCR)and Western blotting were performed to detect the expressions of ferroptosis-related genes and proteins.Results UCMSCmiR-486 markedly reduced radiation-induced apoptosis and especially reactive oxygen species(ROS)levels in AC16 cardiomyocytes and restored both mitochondrial membrane potential and membrane permeability.Intravenous administration of UCMSCmiR-486 via the tail vein significantly increased ejection fraction and shortening fraction in irradiated mouse hearts,decreased the area of myocardial fibrosis,and mitigated mitochondrial ultrastructural damage UCMSCmiR-486 could inhibit ferroptosis of radiated cardiomyocytes and cardiac tissues of mice by modulating the expressions of ferroptosis-related markers,especially acyl-CoA synthetase long chain family member 4(ACSL4)and glutathione peroxidase 4(GPX4).Conclusion UCMSCmiR-486 can mitigate RIHD by inhibiting ferroptosis.This finding is expected to offer a new strategy for the clinical translation of mesenchymal stem cells.