芪红胶囊对冠状动脉微血管疾病小鼠血管内皮细胞氧化应激的影响OA

Objective To investigate the effect of Qihong capsules on mitochondrial oxidative stress in mice with coronary microvascular disease(CMVD)via the TLR4/NF-κB/GPX4 path-way.Methods Twenty CMVD model mice were randomly assigned to 2 groups:model group and Qihong capsules treatment group[0.648 g/(kg·d),intragastric administration for 4 weeks],and additional 10 mice were served as the control group.Hematoxylin-eosin(HE)staining,oil red O staining,and immunohistochemistry were employed to assess coronary pathology and macrophage infiltration.Western blotting and enzyme-linked immunosorbent assay(ELISA)were utilized to de-tect the expression of relevant proteins and levels of reactive oxygen species(ROS).Results Com-pared with the control group,the model group exhibited severe coronary artery injury,elevated ex-pression of inflammatory cytokines(TNF-α,IL-1β,IL-6),enhanced activation of the TLR4/NF-κB pathway,downregulated expression of GPX4,Sirt1,and HO-1,and increased ROS levels(all P<0.001).Following intervention with Qihong capsules,the aforementioned pathological changes were significantly ameliorated,inflammation and oxidative stress were suppressed,and the expression of protective proteins was upregulated(all P<0.001).Conclusion Qihong capsules can alleviate oxidative stress by inhibiting the TLR4/NF-κB inflammatory pathway and upregulating the expression of GPX4/Sirt1/HO-1,thereby improving microvascular dysfunction in CMVD mice.Its mechanism may be associated with the inhibition of ferroptosis,reflecting its potential for multi-target intervention.