七叶皂苷钠改善溃疡性结肠炎大鼠肠黏膜屏障损伤机制研究OA

Objective To explore the effect of sodium aescinate on intestinal mucosal barrier in rats with ulcerative colitis(UC).Methods A total of 65 SD rats were divided into 6 groups:sham operation group,model group,low-dose,medium-dose and high-dose sodium aescinate groups(2.5,5,10 mg/kg),and positive control group(mesalazine,360 mg/kg),10 rats in each group.UC rat models were induced by DSS.Disease activity index(DAI)scores were recorded af-ter drug intervention.The levels of serum D-lactic acid(D-LA),diamine oxidase(DAO),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and IL-6 were detected.The ulcer area,length and weight of colon,expression levels of myeloperoxidase(MPO),Occludin,zonula oc-cludens-1(ZO-1),nuclear factor-erythroid 2-related factor 2(Nrf-2),antioxidant response ele-ment(ARE)and heme oxygenase-1(HO-1)proteins in colon tissues were compared among dif-ferent groups.HE staining was performed and pathological scores were recorded.Results Medium-dose and high-dose sodium aescinate significantly reduced DAI score and pathological score,allevi-ated colonic ulcers,reduced colon weight and increased colon length(P<0.05).The levels of ser-um D-LA,DAO,TNF-α,IL-1β,IL-6,and colonic MPO were significantly reduced,and the expression of Occludin,ZO-1,p-Nrf2/Nrf2,ARE and HO-1 proteins in colonic tissues was upreg-ulated in the medium-and high-dose sodium aescinate groups(P<0.05).Conclusion Sodium aescinate can ameliorate intestinal mucosal barrier dysfunction in DSS-induced UC rats,and the mechanisms may be related to the inflammation and Nrf-2/ARE/HO-1 signaling pathway.