中医湿证的肠道微生态特征的探索性研究OA

Objective To investigate the clinical characteristics,blood biochemical indices,gut microbiota fea-tures,and their interrelationships in individuals with dampness syndrome as defined by traditional Chinese medicine(TCM),and to provide a modern biomedical interpretation of the biological basis of dampness syndrome.Methods Participants undergoing routine health examinations at a health management center were enrolled.TCM dampness syn-drome scores were assessed,and fecal and blood samples were collected.Shotgun metagenomic sequencing was applied to analyze gut microbiota composition,and standard blood biochemical tests were performed.Participants were divided into a dampness syndrome group and a non-dampness syndrome group,and intergroup differences were systematically com-pared.Results A total of 285 participants were included,comprising 144 individuals with dampness syndrome and 141 without.Phenotypic and biochemical analyses revealed that individuals with dampness syndrome tended to be younger and obese.White blood cell count(OR=1.27)and platelet count(OR=1.01)were identified as independent risk factors for dampness syndrome.Gut microbiota analysis demonstrated that Escherichia coli,Klebsiella pneumoniae,and other op-portunistic pathogens were characteristic taxa in the dampness syndrome group,whereas beneficial bacteria such as Bifidobacterium longum and Bifidobacterium adolescentis were enriched in the non-dampness group.A random forest model integrating phenotypic and microbiota data improved the predictive performance for dampness syndrome(AUC=0.769).Mediation analysis further indicated that Ruminococcus_sp_AF13_28 influenced dampness syndrome scores by in-creasing alanine aminotransferase levels.Conclusion The development of TCM dampness syndrome is closely associated with obesity,a state of low-grade inflammation,gut microbiota dysbiosis,and metabolic dysfunction.Gut microbiota may contribute to the occurrence and progression of dampness syndrome by modulating inflammatory and metabolic parameters in the blood.