葛菊功能饮料的抗氧化活性及解酒功效研究OA
Study on antioxidation and alcohol-detoxification function of Ge-Ju functional beverage
评价一种基于葛花菊花解酒古方的功能饮料(简称为"葛菊功能饮料")的抗氧化活性和解酒功效,并考察两者与总黄酮含量的相关性.利用DPPH法、ABTS法、水杨酸法及其对ADH和ALDH的激活率,研究葛菊功能饮料的抗氧化活性和体外解酒功效;采用紫外分光光度法对葛菊功能饮料及不同对照溶液进行总黄酮含量测定,采用SPSS分析软件考察总黄酮含量与抗氧化活性和解酒功效的相关性.结果显示,葛菊功能饮料有较强的清除自由基及激活ADH及ALDH的活性,将不同溶液对DPPH自由基、ABTS自由基、羟基自由基的清除率及对ALD和ALDH的激活率与溶液中总黄酮含量进行相关性分析,相关系数依次为 0.668、0.759、0906、0.777 和0.723,说明总黄酮含量与抗氧化活性和解酒功效均呈现出较强的正相关.以上表明该功能饮料具有潜在的解酒功效,具有一定的开发价值.
This study aims to evaluate the antioxidant activity in vitro and alcohol-detoxification function of an ancient prescription-based functional beverage(Ge-Ju functional beverage,formulated with Puerariae Flos and Chrysanthemum for alcohol relief),and to explore the correlation between its activities and the total flavonoid content.DPPH method,ABTS method and salicylic acid method were used to study the antioxidant activity of the functional beverage in vitro.By investigating the activation of athanol dehydrogenase(ADH)and acetaldehyde dehydrogenase(ALDH)of the functional beverage,the alcohol-detoxification function in vitro was studied.The total flavonoid content of the functional beverage and different control solutions were determined by ultraviolet spectrophotometry,and the correlation between the total flavonoid content and the antioxidant activity and alcohol-detoxification function activity was examined using SPSS analysis software.The results have shown that the Ge-Ju functional beverage not only has the antioxidant effect of scavenging free radicals,but also has the effect of activating ADH and ALDH.Correlation analysis was conducted between the total flavonoid content in different solutions and their scavenging rates against DPPH,ABTS,and hydroxyl radicals,as well as their activation rates on ALD and ALDH.The correlation coefficients were 0.668,0.759,0.906,0.777,and 0.723,respectively,indicating a strong positive correlation between total flavonoid content and both antioxidant activity and alcohol?detoxifying efficacy.Therefore,the functional beverage has potential effects of relieving alcohol intoxication and protecting the liver,and it has certain development value.
权春梅;李媛媚;王昭耀;凌庆龙
亳州职业技术学院药学院,安徽亳州 236800亳州职业技术学院药学院,安徽亳州 236800安徽中医药大学药学院,安徽 合肥 230000安徽凌氏药业有限公司,安徽亳州 236800
轻工纺织
葛菊功能饮料抗氧化活性解酒功效总黄酮含量相关性分析
Ge-Ju functional beverageantioxidant activityalcohol-detoxification functiontotal flavonoid contentcorrelation analysis
《佛山科学技术学院学报(自然科学版)》 2026 (1)
24-30,7
2023年安徽省高校自然科学研究重点项目(2023AH053217)2024年安徽省高校自然科学研究重点项目(2024AH051318)
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