猪繁殖与呼吸综合征病毒糖蛋白2a的原核表达及多克隆抗体制备OA

To generate polyclonal antibodies against Glycoprotein 2a(GP2a)of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV),the recombinant GP2a protein was expressed in a prokaryotic system using the genome of the highly pathogenic XH-GD strain as a template.The purified recombinant GP2a was used to immunize rabbits to produce rabbit polyclonal antibodies.The antiserum was subsequently evaluated for its titer and specificity.The results showed that the obtained polyclonal antibodies had a titer of 1∶12,800 and could specifically react with eukaryotic-expressed Flag-GP2a.This indicates that the prokaryotically expressed recombinant GP2a protein possesses good immunogenicity,and the resulting antibodies exhibit high titer and desirable specificity.These findings lay a material foundation for further investigation into the function of PRRSV GP2a.