NF-YC15转录因子调控木薯抗细菌性枯萎病的互作蛋白筛选及功能验证OA

[Objective]Molecular mechanism of NF-YC15 transcription factor associated with the regulation of bacterial blight resistance of Manihot esculenta was investigated.[Methods]Yeast two-hybrid was employed to determine the interacting proteins with the MeNF-YC15 transcription factor.Bimolecular fluorescence complementation and pull-down experiments were conducted to verify the interactions.On overexpressed cassava,bacterial blight pathogens were inoculated to study disease resistance of the plant.Through transcriptome sequencing,downstream disease-resistant genes regulated by MeNF-YC15 were identified,and the molecular mechanism analyzed by quantitative PCR.[Results]The key inhibitor of jasmonic acid ZIM domain protein MeJAZ2.2 in the signal transduction pathway was identified to be the sole protein interacting with MeNF-YC15,which was further verified in vivo and in vitro in plants.The bacteria and disease lesion areas on the significantly taller MeNF-YC15-overexpressed plants were significantly smaller than those on the MeNF-YC15-MeJAZ2.2-co-overexpressed counterparts and control.MeNF-YC15 activated the downstream disease resistant WRKY68,matrix metalloproteinase-3(MMP-3),and leucine-rich repeat receptor-like kinases(LRR-RLK)of jasmonic acid,and in contrast,MeJAZ2.2 inhibited the expressions.[Conclusion]MeNF-YC15 and MeJAZ2.2 antagonistically regulated the expression of downstream disease-resistant genes of jasmonic acid and the resistance of cassava plant to the bacterial blight through the jasmonic acid pathway.