裂壶藻粉和维生素 E对绒山羊体外瘤胃发酵、营养物质降解及脂肪酸组成的影响OA

This study investigated the effects of dietary supplementation with Schizochytrium powder(SH)and vitamin E(VE),either individually or in combination,on in vitro ruminal fermentation,nutrient degradability and fatty acid composition in cashmere goats.Rumen fluid was collected orally from three 1.0-1.5 year-old Al-bas white cashmere goats[body weight:(45±3)kg].A 2×3 factorial completely randomized design was em-ployed,with two levels of SH(0 and 1.5%,designated as SH0 and SH1.5,respectively)and three levels of VE(0,250 and 500 IU/kg,designated as VE0,VE1 and VE2,respectively).This resulted in six treat-ments:SH0+VE0(basal diet,no SH and VE),SH0+VE1(basal diet+250 IU/kg VE),SH0+VE2(basal diet+500 IU/kg VE),SH1.5+VE0(basal diet+1.5%SH),SH1.5+VE1(basal diet+1.5%SH+250 IU/kg VE),and SH1.5+VE2(basal diet+1.5%SH+500 IU/kg VE).Each treatment had six replicates.The results showed as follows:compared with SH0,SH1.5 significantly increased gas production,the contents of bacterial crude protein(BCP),propionic acid,butyric acid,and isovaleric acid,as well as the degradation rates of crude protein(CP),ether extract(EE)and neutral detergent fiber(NDF)(P＜0.05),while significantly de-creasing the contents of ammonia nitrogen(NH3-N)and acetic acid,and the acetic acid/propionic acid ratio(P＜0.05).Compared with VE0,VE1 significantly improved the CP degradation rate(P＜0.05).Among the multiple combination effect indices,SH1.5+VE0 showed the best performance,followed by SH1.5+VE1.Both SH1.5 and VE1,VE2 significantly increased the contents of C18∶3n3,C20∶5n3,C22∶6n3,polyunsatu-rated fatty acids(PUFA),n-3 PUFA,n-3 long-chain polyunsaturated fatty acids(n-3 LCPUFA),and the PUFA/saturated fatty acid(P/S)in the in vitro rumen fermentation fluid(P＜0.05);SH1.5 also significantly increased the content of C18∶1n9c(P＜0.05)and significantly decreased the content of C18∶0 and the n-6 PUFA/n-3 PUFA(P＜0.05).There were significant interactive effects between SH and VE on the contents of C18∶3n3,C20∶5n3,C22∶6n3,PUFA,n-3 PUFA,n-3 LCPUFA and the P/S(P＜0.05),with the combina-tions of SH1.5+VE1 and SH1.5+VE2 being more favorable.In conclusion,dietary supplementation of 1.5%SH can promote in vitro rumen fermentation and nutrient degradation in cashmere goats;both 1.5%SH alone and 250 or 500 IU/kg VE alone can improve the PUFA composition,increase the contents of C22∶6n3,C18∶3n3,C20∶5n3,and n-3 PUFA,and the combined supplementation of the two can further enhance the contents of the aforementioned fatty acids.[Chinese Journal of Animal Nutrition,2026,38(3):2294-2305]