硒对驴外周血单个核细胞中 1-磷酸-鞘氨醇的合成及其介导的抗氧化和抗炎症反应的促进作用OA

This study was conducted to investigate the promoting effects of selenium(Se)on the synthesis of sphingosine-1-phosphate(S1P)and its mediated antioxidant and anti-inflammatory responses in peripheral blood mononuclear cells(PBMCs)of donkey,and to screen for the appropriate doses of Se or S1P.Two ex-periments were employed a single-factor completely randomized experimental design,adding different doses of Se[0,0.2,0.5,1.0,2.0 and 5.0 nmol/L,which were named as SE0(control),SE0.2,SE0.5,SE1.0,SE2.0 and SE5.0 groups,with eight replicates per group]or different doses of S1P[0,0.1,0.2,0.5,1.0 and 2.0 nmol/L,which were named as SP0(control),SP0.1,SP0.2,SP0.5,SP1.0 and SP2.0 groups,with eight replicates per group]in PBMCs.The results showed as follows:1)compared with SE0 group,the cell viability and activities of catalase(CAT),glutathione peroxidase(GPx)and superoxide dismutase(SOD)and contents of S1P and interleukin(IL)-2 in PBMCs of SE0.5 and SE1.0 groups were significantly increased(P＜0.05),and the contents of IL-4,IL-6 and lipopolysaccharide(LPS)were significantly decreased(P＜0.05);the mRNA relative expression levels of sphingosine-1-phosphate receptor 2(S1PR2),acid ceramidase 1(ASAH1)and sphingosine kinase 2(SPHK2)in PBMCs of SE0.5 and SE1.0 groups were significantly in-creased(P＜0.05);the mRNA relative expression levels of IL-2 and IL-10 in PBMCs of SE1.0 group were significantly increased(P＜0.05),and the IL-6 mRNA relative expression level was significantly decreased(P＜0.05).2)Compared with SP0 group,the activities of CAT and SOD and contents of IL-2 and IL-6 in PBMCs of SP0.2 and SP0.5 groups were significantly increased(P＜0.05),and the content of malondialde-hyde(MDA)and activities of inducible nitric oxide synthase(iNOS)and reactive oxygen species(ROS)were significantly decreased(P＜0.05);the mRNA relative expression levels of S1PR2,ASAH1,SPHK2 and IL-10 in PBMCs of SP0.2 group were significantly increased(P＜0.05);the mRNA relative expression levels of GPx1 and IL-2 in PBMCs of SP0.2 and SP0.5 groups were significantly increased(P＜0.05),and the mRNA relative expression levels of tumor necrosis factor-α(TNF-α)and IL-6 were significantly decreased(P＜0.05).In summary,both Se and S1P can promote the antioxidant level and anti-inflammatory response in PBMCs of donkey,enhance the cell vitality,and Se can increase the S1P content and the mRNA relative ex-pression levels of S1P synthesis-related genes ASAH1 and SPHK2 and receptor gene S1PR2.It shows good effects when the addition doses of Se and S1P are 0.5,1.0 nmol/L and 0.2,0.5 nmol/L,respectively.[Chi-nese Journal of Animal Nutrition,2026,38(3):2184-2194]