饲粮中添加椰子油对脂多糖诱导的仔猪肌肉损伤和环鸟苷酸-腺苷酸合成酶-干扰素基因刺激因子信号通路的影响OA

This experiment aimed to investigate the effects of dietary coconut oil supplementation on lipopo-lysaccharide(LPS)-induced muscle injury and cyclic GMP-AMP synthase(cGAS)-stimulator of interferon gene(STING)signaling pathway in piglets.A total of twenty-four 28-day-old"Duroc×Landrace×Yorkshire"weaned piglets were randomly allocated to four groups with six replicates per group and one piglet per replicate.A 2×2 factorial design was adopted,with the main factors being dietary treatment(supplemented with 3%soy-bean oil or 3%coconut oil)and LPS treatment(injected with LPS or saline).On day 28 of the trial,piglets were intraperitoneally injected with 100 μg/kg BW LPS or an equal volume of saline,and samples were col-lected after slaughter 4 hours later.The results showed as follows:1)coconut oil significantly reduced the ser-um total protein(TP)content under LPS challenge(P＜0.05),and alleviated the LPS-induced decrease in ser-um glucose(GLU)content(P＞0.05).2)Coconut oil significantly exacerbated the LPS-induced increase in the mRNA relative expression level of interleukin-6(IL-6)in muscle(P＜0.05),and significantly inhibited the LPS-induced increase in the mRNA relative expression level of interleukin-10(IL-10)in muscle(P＜0.05).3)Coconut oil significantly reduced the muscle malondialdehyde(MDA)content(P＜0.05)and sig-nificantly alleviated the LPS-induced increase in muscle total antioxidant capacity(T-AOC)(P＜0.05).4)Coconut oil significantly increased the mRNA relative expression level of forkhead box protein O1(FOXO1)in muscle(P＜0.05),and significantly increased the relative mRNA expression of both FOXO1 and forkhead box protein O4(FOXO4)in muscle under LPS challenge(P＜0.05).5)Coconut oil significantly increased the mRNA relative expression level of 2′,5′-oligoadenylate synthetase 2(OAS2)in muscle(P＜0.05),and significantly exacerbated the LPS-induced increase in the mRNA relative expression level of STING in muscle(P＜0.05).Based on the above results,it can be concluded that replacing an equal amount of soybean oil with coconut oil in the diet promotes the activation of the cGAS-STING signaling pathway,and exacerbates LPS-in-duced muscle inflammation and protein degradation in piglets.[Chinese Journal of Animal Nutrition,2026,38(3):2164-2172]