平菇多糖通过靶向线粒体功能障碍缓解黄曲霉毒素B1诱导的猪小肠上皮细胞氧化损伤OA

This study aimed to investigate the alleviating effect of Pleurotus ostreatus polysaccharides(POP)on aflatoxin B1(AFB1)-induced oxidative damage in porcine intestinal epithelial cells(IPEC-J2 cells).First,the working concentrations of POP and AFB1,as well as the pretreatment time of POP,were determined using the CCK-8 kit.Subsequently,IPEC-J2 cells were divided into four groups(with three replicates per group):1)Control group,no treatment;2)AFB1 group,treated with 16 μmol/L AFB1 for 24 h;3)POP+AFB1 group,pretreated with 0.25 mg/mL POP for 24 h,followed by co-treatment with 16 μmol/L AFB1 for anoth-er 24 h;4)POP group,treated with 0.25 mg/mL POP for 24 h.After treatment,cells were collected to meas-ure reactive oxygen species(ROS)relative content,mitochondrial membrane potential,ATP relative level,antioxidant enzyme activities,and the relative expression levels of apoptosis-related proteins and mitochondrial dynamics-related proteins.The results showed that:1)treatment of IPEC-J2 cells with 16 μmol/L AFB1 for 24 h successfully established an oxidative damage model;pretreatment with 0.25 mg/mL POP for 24 h ex-tremely significantly increased cell viability(P＜0.01).2)Compared with the Control group,the AFB1 group showed an extremely significant increase in ROS relative content(P＜0.01),an extremely significant decrease in mitochondrial membrane potential and ATP relative level(P＜0.01),an extremely significant reduction in the relative expression level of mitofusin 1(MFN1)(P＜0.01),a significant reduction in the relative expres-sion level of mitofusin 2(MFN2)(P＜0.05),an extremely significant decrease in the relative expression level of the anti-apoptotic protein B-cell lymphoma-2(Bcl-2)(P＜0.01),a significant decrease in the Bcl-2/Bcl-2-associated X protein(Bax)ratio(P＜0.05),and an extremely significant reduction in the activities of the an-tioxidant enzymes glutathione peroxidase(GPX),superoxide dismutase(SOD)and catalase(CAT)(P＜0.01).In contrast,pretreatment with 0.25 mg/mL POP for 24 h reversed all the above changes.In conclu-sion,POP alleviates AFB1-induced oxidative damage in porcine intestinal epithelial cells by restoring mitochon-drial function and maintaining mitochondrial dynamics homeostasis.[Chinese Journal of Animal Nutrition,2026,38(3):2154-2163]