基于网络药理学探讨五味子乙素抑制胰腺癌AKT通路OA

Schisandrin B(SchB)is the most abundant dibenzocyclooctadiene lignan in Chinese magnolia vine fruit,but research on its role in pancreatic cancer,which has an extremely poor prognosis,remains limited.To further investigate the mechanism by which SchB inhibits pancreatic cancer,experiments are conducted using pancreatic cancer PANC-1 cells as a model.MTT assays show that SchB inhibits cell proliferation in a concentration-dependent manner;when combined with gemcitabine(GEM),it exhibits significant chemosensitization effects;Transwell invasion assays confirm that the drug effectively suppresses cell invasion.Based on these findings,network pharmacology is further employed to predict the molecular mechanisms and biological functions of SchB in pancreatic cancer.Western Blot analysis demonstrates that SchB inhibits the AKT pathway;comet assay results confirm that it promotes DNA damage,thereby verifying the predictions of network pharmacology.In summary,the findings indicate that SchB has the potential to become a therapeutic agent for pancreatic cancer or a sensitizer for gemcitabine.