火烧法胁迫下棋楠沉香品质及功能活性评价OA

Aquilaria sinensis was induced by treating A.sinensis with three fire-burning durations,namely 45 s(SW),60 s(LW),and 75 s(QW).After 12 months of treatment,A.sinensis samples were collected to determine the yields of ethanol extracts and essential oils,conduct color reaction identification,and analyze their chemical compositions by gas chromatog-raphy-mass spectrometry(GC-MS).Furthermore,the samples'scavenging capacities against DPPH and ABTS+free radi-cals as well as their anti-acetylcholinesterase activity were evaluated.The results showed that:(1)The mass fractions of ethanol extracts in the SW,LW,and QW groups were(54.27±0.89)％,(56.14±1.08)％,and(61.37±1.81)％respec-tively,while the mass fractions of essential oils were(41.62±1.02)％,(42.20±1.63)％,and(46.87±0.77)％respec-tively.(2)All samples exhibited cherry red or violet color in the color reactions,which met the requirements for physico-chemical color reactions specified in the Pharmacopoeia of the People's Republic of China.(3)A total of 89 compounds were identified.The main components in each treatment group were 2-(2-phenylethyl)chromones(PECs),sesquiterpe-noids,and aromatic compounds.The numbers of these three types of compounds in SW,LW,and QW were 33,43,and 35 respectively,with the total relative mass fractions reaching 94.73％,95.65％,and 96.22％respectively.In contrast,the control group(CK)was dominated by aliphatic and aromatic compounds.(4)In terms of bioactivity,the half-maximal inhibitory concentrations(IC50)of SW,LW,and QW against DPPH free radicals were 0.286 2,0.259 8,and 0.157 2 g/L respectively,their IC50 values against ABTS+free radicals were 0.549 0,0.347 7,and 0.107 8 g/L respectively,and their IC50 values against acetylcholinesterase were 1.916 8,1.337 5,and 1.167 0 g/L respectively.In conclusion,fire-burning stress can significantly improve the agarwood-inducing efficiency and quality of A.sinensis,among which the QW(75 s)treatment showed the best performance in extract yield,characteristic component content,and bioactivity.To fur-ther clarify the critical effect of fire-burning duration on agarwood quality,subsequent studies can add experimental groups with longer treatment durations(e.g.,90 s and 105 s)to reveal the threshold of component accumulation and optimal stress conditions.The fire-burning method is an efficient and high-quality agarwood-inducing approach,which has important ap-plication value for promoting the high-quality production of agarwood.