青贮玉米茎腐病拮抗真菌的筛选及盆栽防治效果OA

[Objective]To effectively prevent and control silage corn stalk rot,twenty-seven biocontrol fungi ob-tained from primary screening were used as research objects to further screen high-efficiency biocontrol fungi.[Method]Biocontrol fungi with high antagonism activity against corn stalk rot were screened by plate confrontation method.The species of biocontrol fungi were identified through morphological observations and molecular biological analysis.Furthermore,the control effect on corn stalk rot and the growth-promoting effects of the candidate biocontrol fungi were evaluated via pot experiments.[Result]A specific strain,SL-5,with efficient antagonism against corn stalk rot was screened,and the inhibition rate was 56.46%.It was identified as Trichoderma koningiopsis based on morphological and molecular biological characteristics.The results of pot experiments showed that the biocontrol ef-fect of T.koningiopsis SL-5 fermentation liquid on corn stalk rot was 54.21%,and it had a significant growth-pro-moting effect on corn seedlings.[Conclusion]T.koningiopsis SL-5 screened in this experiment exhibits certain con-trol effects against corn stalk rot and demonstrates growth-promoting properties on maize seedlings,indicating poten-tial application value.