苇状羊茅种子表型性状遗传多样性分析与综合评价OA

[Objective]To provide a scientific basis for the collection,evaluation,and utilization of Festuca arun-dinacea germplasm resources.[Method]By using the methods of correlation analysis,principal component analysis(PCA),cluster analysis and membership function,five seed phenotypic traits(seed length,seed width,1000-seed weight,seed aspect ratio and single seed area)of 73 F.arundinacea germplasm resources were comprehensively ana-lyzed.[Result]There were differences in the degree of genetic variation among the five phenotypic traits,with the largest coefficient of variation observed in 1000-seed weight.PCA revealed that the contribution rates of the first two principal components were 58.959%and 28.364%,respectively,with a cumulative contribution rate of 87.323%,which encompassed all the information on the seed traits of the tested materials.Correlation analysis showed that there was a very significant positive correlation between 1000-seed weight and seed length,width,and single-seed area(P<0.01).There was also a very significant positive correlation between single-seed area and seed length,width,and length-to-width ratio(P<0.01),as well as between length-to-width ratio and seed length(P<0.01).Cluster analysis divided the 73 materials into three major groups.Group I contained 2 germplasm materials,Group II contained 1 germplasm material,and Group III included 70 germplasm materials.Comprehensive evaluation using the membership function method identified five germplasm materials(LF-73,LF-15,LF-28,LF-27,LF-47)with superior overall performance.[Conclusion]Seed length is the most critical factor influencing the genetic diversity of F.arundinacea germplasm.The variation in seed length and 1000-seed weight can provide reference for the identification and cultivar breeding of F.arundinacea germplasm resources.