马尾松枯梢病病原拮抗真菌的筛选与鉴定OA
Screening and identification of antagonistic fungi to Diplodia sapinea,the pathogen of Pinus massoniana shoot blight
[目的]筛选获得对马尾松枯梢病菌松色二孢具有良好抑菌效果的拮抗真菌,并对其进行鉴定,为马尾松枯梢病的生物防治提供理论依据.[方法]以重庆马尾松枯梢病患病树干和树梢韧皮部样本上分离纯化的病原真菌松色二孢和 4 株拮抗真菌为材料,采用平板对峙培养法和菌丝生长速率法筛选病原真菌松色二孢的拮抗真菌,结合形态学和系统发育学分析对拮抗真菌进行鉴定,并通过显微观察松色二孢和拮抗真菌菌丝之间相互作用方式,初步探究其生防机制.[结果]初筛结果表明,4株拮抗真菌对松色二孢均有抑菌效果,其中,抑菌效果最好的是菌株CXY4103(毛色二孢属)和CGMCC 41126(木霉属).复筛结果表明,菌株CGMCC 41126 对松色二孢抑制效果最好,其发酵滤液的抑菌率为 45.17%.通过形态学观察与tef1和rpb2 基因序列分析,鉴定菌株CGMCC 41126 为近渐绿木霉.显微形态观察发现,近渐绿木霉可与松色二孢的菌丝发生接触、缠绕.抑菌谱结果表明,近渐绿木霉对长喙壳类真菌双色长喙壳、拟双色长喙壳、富士长喙壳、E.polonica、栎叶斑病病原菌和板栗溃疡病病原菌均具有较好的拮抗效果.[结论]筛选获得 1株对马尾松枯梢病菌松色二孢具有良好抑菌效果的拮抗真菌,经鉴定为近渐绿木霉,其抑菌谱广泛,为马尾松枯梢病的生物防治提供了潜在拮抗菌源和防控依据.
[Objective]This study aimed to screen and identify the antagonistic fungi with strong inhibitory effects against Diplodia sapinea,the pathogen of Pinus massoniana shoot blight,to provide a theoretical basis for the biocontrol of P.massoniana shoot blight.[Method]Strains of D.sapinea isolated and purified from the phloem of diseased trunks and shoots of P.massoniana in Chongqing of southwestern China and the four fungal antagonistic strains were used as materials.The plate confrontation culture and mycelial growth rate methods were employed to screen for antagonistic fungi against D.sapinea.The antagonistic fungi were identified based on morphology and phylogenetic analysis,and the biocontrol mechanism was preliminarily explored by microscopic observation of hyphal interactions between D.sapinea and the antagonistic fungi.[Result]The results of preliminary screening showed that all four fungal antagonistic strains inhibited the growth of D.sapinea,among which strains CXY4103(Lasiodiplodia sp.)and CGMCC 41126(Trichoderma sp.)were the most effective one.The results of re-screening revealed that the strain CGMCC 41126 had the best inhibitory effect on D.sapinea,with its fermentation filtrate achieving an inhibition rate of 45.17%against the pathogen.Through morphological observation and phylogenetic analysis of tef1 and rpb2 gene sequences,the strain CGMCC 41126 was identified as Trichoderma paraviridescens.According to the microscopic observation,T.paraviridescens could contact and coil around the mycelia of D.spinea.The antifungal spectrum result indicated that T.paraviridescens had antagonistic effect on Ophiostoma bicolor,O.pseudobicolor,Endoconidiophora fujiensis,E.polonica,Gnomoniopsis daii and G.chinensis.[Conclusion]A strain of antagonistic fungus with strong inhibitory effect against D.sapinea,the pathogen of P.massoniana shoot blight was screened and identified as T.paraviridescens.Its antifungal spectrum is extensive.This study provides a potential antagonistic fungal resource and a theoretical basis for the biocontrol of P.massoniana shoot blight.
刘端冲;李晓菲;王甜甜;王慧敏;刘会香;吕全
中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林保护学重点实验室,北京 100091中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林保护学重点实验室,北京 100091||山东农业大学植物保护学院,山东 泰安 271018中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林保护学重点实验室,北京 100091中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林保护学重点实验室,北京 100091山东农业大学植物保护学院,山东 泰安 271018中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林保护学重点实验室,北京 100091
农业科技
马尾松枯梢病松色二孢拮抗真菌筛选鉴定近渐绿木霉
Pinus massonianashoot blightDiplodia sapineaantagonistic fungiscreeningidentificationTrichoderma paraviridescens
《北京林业大学学报》 2026 (3)
53-62,10
国家重点研发计划(2022YFD1401005),中国林业科学研究院基本科研业务费专项(CAFYBB2023XA001).
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