基于转录组和SNP标记研究草珊瑚群体遗传分化OA

The extensive collection of Sarcandra glabra by the pharmaceutical industry necessitates a re-evaluation of the wild germplasm resources for the traditional medicinal herb.However,there have been no studies on large-scale S.glabra populations based on single nucleotide polymorphisms(SNPs)to date.In this study,the transcriptomes of 16 collected individuals were sequenced,and 7 605 high-quality SNPs were developed.Based on the analyses of SNPs with three different methods,the samples were divided into two distinct groups,namely the temperate and tropical groups.Between the two groups,2 326 genes demonstrated significant variations in allele frequency,2 460 genes were differentially expressed,and this intersection of the two gene sets contains 19 genes.A subset of the SNPs were validated by using MassARRAY with a call rate of 61.9％,which can be utilized for further exploration and conservation management of S.glabra.A significant correlation between genetic distance and geographical distance among different samples suggests that the genetic differentiation may primarily be attributed to geographical isolation.